慢性非细菌性前列腺炎患者前列腺液革兰阴性菌16SrRNA基因的检测

目的　探讨慢性非细菌性前列腺炎的可能病因。　方法　应用PCR方法检测 2 4例慢性非细菌性前列腺炎患者和 10例正常人前列腺液中革兰阴性菌 16SrRNA基因。同时对 10例实验组患者行尿道分泌物PCR检查。　结果　 2 4例慢性非细菌性前列腺炎患者前列腺液中革兰阴性菌16SrRNA基因阳性 13例 ,阳性率 5 4 % ;10例正常人中阳性 1例 ,阳性率 10 % (P <0 .0 1)。 10例慢性非细菌性前列腺炎尿道分泌物PCR均阴性。　结论　慢性非细菌性前列腺炎患者前列腺液革兰阴性菌 16SrRNA基因阳性率显著高于正常人。革兰阴性菌 16SrRNA基因源于前列腺而非尿道。一些目前还不能常规成功培养的细菌可能为慢性非细菌性前列腺炎的发病原因之一。

Assay of gram-negative bacteria 16SrRNA gene in chronic abacterial prostatitis

Objective To explore the possible etiological factors in chronic abacterial prostatitis. Methods 16SrRNA gene of gram-negative bacteria was assayed in 10 urethral swab and 24 EPS samples from the patients with chronic abacterial prostatitis.Also,samples from 10 normal male adults (controls) EPS were assayed by the same technique. Results Among the 24 chronic abacterial prostatitis EPS samples,16SrDNA was positive in 13.But the result was negative in all the urethral swab samples.Only one of the 10 normal EPS was positive for 16SrRNA gene( P <0.01). Conclusions 16SrRNA gene is more easily detected in patients with chronic abacterial prostatitis than in normal adults.16SrRNA gene of gram-negative bacteria detected come from prostate,not from urethra.Some traditionally unculturable bacteria may be an etiological factor in chronic abacterial prostatitis.