胚胎脊髓移植对大鼠损伤脊髓胶质纤维酸性蛋白表达的影响

目的 研究大鼠脊髓损伤后胚胎脊髓（ＦＳＣ）移植是否能够影响胶质纤维酸性蛋白（ＧＦＡＰ）的表达和伤后大鼠后肢功能的恢复情况。方法 将６０只大鼠分为脊髓半脊髓半切洞损伤＿胚胎脊髓移植组（Ａ组）和单纯脊髓半切洞损伤组（Ｂ组）。伤后１，３，，７，１４，２８天，应用行为学和电生理检查观察大鼠功能恢复情况，应用原位杂我和免疫细胞化学方法观察ＧＦＡＰ的表达，并采用计算机图像分析技术，进行定量分析。结果 大鼠脊髓

Effect of fetal spinal cord transplantation on graft glia fibrillary acidic protein expression after spinal cord injury in rats

Objective To investigate whether fetal spinal cord (FSC) transplantation effected the expression of the glia fibrillary acidic protein (GFAP) mRNA and immunoreaction of GFAP as well as functional recovery after spinal cord injury in rats. Methods Experimental rats were divided into 2 groups, spinal cord hemisection injury combination with transplantation of FSC to injured site group (Group A), spinal cord injury and recept of gelfoam pledgets group (Group B). Rats were killed at 1, 3, 7, 14, 28 days. Then, open field and inclined plane tests, somatosensory evoked potentials(SEP) and motor evoked potentials(MEP) were examined. An in situ hybridization and immunocytochemistry was used to examine the spinal cord lesion and neighbor areas. The positive cells were quantitatively analysed by a computer image analysis system. Results In Group A, the number of cell expression protein GFAP mRNA and GFAP immunoreaction was more than that in Group B. By 28 days following injury, positive astrocytes cell of Group A returned to normal level but, the positive cells increased by 14 days postinjury in Group B. These increases in GFAP positive cells were paralleled by a significant improvement in neurological function recovery. Conclusions FSC transplantation is sufficient to reduce multiple aspects of gliosis and can promote neurological function recovery of adult rats after spinal cord injury.