IL-10-592 polymorphism is associated with IL-10 expression and severity of enterovirus 71 infection in chinese children |
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| Affiliation: | 1. Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Shimotsuke, Tochigi 329-0498, Japan;2. Department of Internal Medicine, Kiryu Kosei General Hospital, Kiryu, Gunma 376-0024, Japan;3. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan;4. Department of Gastroenterology and Hepatology, Mie University School of Medicine, Tsu, Mie 514-8507, Japan;5. Department of Internal Medicine, Sakakibara Onsen Hospital, Tsu, Mie 514-1293, Japan;6. Department of Gastroenterology, Suzuka General Hospital, Suzuka, Mie 513-8630, Japan;7. Department of Internal Medicine, Suzuka Kaisei Hospital, Suzuka, Mie 513-8505, Japan;8. Department of Internal Medicine, Fujita Health University Nanakuri Memorial Hospital, Tsu, Mie 514-1295, Japan;9. Department of Gastroenterology/Liver Center, Hiroshima Red Cross Hospital and Atomic-bomb Survivors Hospital, Hiroshima, Hiroshima 730-8619, Japan;10. Department of Internal Medicine/Liver Disease Center, Okayama Saiseikai General Hospital, Okayama, Okayama 700-8511, Japan;1. Department of Medicine, Brigham and Women''s Hospital, Boston, MA 02115, United States;2. Dana Farber Cancer Institute, Boston, MA 02115, United States;3. Broad Institute of MIT and Harvard, Cambridge, MA 02142, United States |
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| Abstract: | BackgroundEnterovirus 71 (EV71) infection results in some severe complications with high mortality and disability in Hand, Foot and Mouth Disease (HFMD) in children. Recent studies have shown that cytokine genetic predispositions have associations with both the development of EV71 infection and severity of HFMD.ObjectiveThis study was designed to investigate whether the IL-10–592 polymorphism is associated with IL-10 levels and disease severity in Chinese children with EV71 infection.Study designIn patients selected, there were 378 cases with EV71 infection (including 291 mild cases, 70 severe cases and 17 critical cases), as well as 406 health controls. EV71 in serum was tested by RT-PCR, and IL-10-592 genotype was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis techniques.ResultThe IL-10-592C allele was observed with higher frequency in patients with critical EV71 infection (70.59%) compared with severe EV71 infection (41.43%, P < 0.01), mild EV71 infection (43.81%, P < 0.01) and healthy children (44.46%, P < 0.01). The blood IL-10 levels of critical cases were significantly higher than severe cases, mild cases, and healthy children. Among all of the four groups, IL-10 levels in patients with genotype AA were significantly lower than those with genotypes AC + CC (t = 4.86, P < 0.05; t = 2.30, P < 0.05; t = 3.44, P < 0.05; t = 5.58, P < 0.05).ConclusionIL-10-592C allele is associated with IL-10 expressions and the severity of EV71 infection in Chinese patients. |
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| Keywords: | Enterovirus 71 infection IL-10 Gene polymorphism |
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