Human enterovirus D68 in clinical and sewage samples in Israel |
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| Affiliation: | 1. Central Virology Laboratory, Public Health Services, Ministry of Health and Chaim Sheba Medical Center, Tel-Hashomer, Israel;2. Tel Aviv University, Tel-Aviv, Israel;3. Chaim Sheba Medical Center, Tel-Hashomer, Israel;4. Virology Laboratory, Soroka University Medical Center, Beer-Sheva, Israel;5. Clinical Virology Unit, Hadassah Hebrew University Medical Center, Jerusalem, Israel;6. Virology Laboratory, Rambam Health Care Campus, Haifa, Israel;7. Israel Center for Disease Control, Tel-Hashomer, Israel;1. Department of Epidemiology, Geisel School of Medicine, Dartmouth College, Hanover, NH, USA;2. Department of Pediatrics, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA;1. Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John''s University, Queens, NY 11439, USA;2. Departments of Pathology and Obstetrics and Gynecology and Women''s Health, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10461, USA;1. Division of Vaccine Research, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention, Chungcheongbuk-do, 28159, Republic of Korea;2. College of Veterinary Medicine, Konkuk University, Seoul, 05029, Republic of Korea;1. Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium;2. School of Life Sciences, Hasselt University, Agoralaan Building D, 3590 Diepenbeek, Belgium |
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| Abstract: | BackgroundSince mid-August 2014, North America experienced a wide outbreak of Enterovirus D68 (EV-D68) associated with severe respiratory illness in children. Several other countries also reported cases of EV-D68 in 2014.ObjectivesThe aim of this study was to determine whether EV-D68 circulated in Israel in 2014, caused severe respiratory illness in children and was the causative agent of Acute Flaccid Paralysis.Study designArchived clinical respiratory samples from a cohort of 710 hospitalized pediatric patient’s (<10 years old) with respiratory illness were screened for clade B specific EV-D68 by real-time PCR. The patients were seen at four medical centers covering the entire country between August and November 2014. We also evaluated 49 patient stool samples from 26 AFP cases during 2014 for presence of EV-D68. In addition, RNA from sewage samples collected throughout Israel during the same study period was also tested for EV-D68. Partial VP1 sequencing was performed on all positive samples.ResultsOf the 710 clinical samples evaluated, 7 (1%) were positive for EV-D68. Two patients were from the central part of Israel, while the rest was from the southern part. The majority of the patients did not have any underlying disease. Not only that, but, none of the 26 suspected AFP cases had EV-D68 nucleic acid in their stool samples. EV-D68 RNA was detected in 9 out of 93 sewage samples, mainly from Southern Israel. Sequence analysis of EV-D68 VP1 gene from both sewage and clinical samples indicated that the Israeli EV-D68 RNA belonged to Clade B which was genetically similar to 2014 circulating European and North American EV-D68 virus.ConclusionsEV-D68 circulated in Israel during the 2014 summer-fall season and caused hospitalization of a small percent of the patients with respiratory illness. |
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| Keywords: | Enterovirus D68 Respiratory illness VP1 sequencing |
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