Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus |
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Authors: | Maria Assunta Biscotti Marco Barucca Teresa Capriglione Gaetano Odierna Ettore Olmo Adriana Canapa |
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Affiliation: | Istituto di Biologia e Genetica, Facoltà di Scienze, Università Politecnica delle Marche, via Brecce Bianche, I-60131, Ancona, Italy. |
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Abstract: | Two highly repeated DNAs, designated NmE1/NmE2 and NmE5, were identified by EcoRV digestion in the chiton Nuttallochiton mirandus (Mollusca: Polyplacophora). The comparison of the sequences obtained showed high similarity in 5' and 3' regions and the NmE5 sequence displayed an inserted sequence that might arise from a transposable element. Southern blotting analyses suggested a tandem organization of both satellite DNA families identified. Moreover, dot blot analyses, performed on several molluscan species, revealed a different degree of conservation of the repeated DNAs. Fluorescence in-situ hybridizations (FISH) on metaphase chromosomes showed that both satellite DNAs are located at centromeric regions. |
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Keywords: | chiton fluorescence in-situ hybridization Nuttallochiton mirandus satellite DNA |
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