| EB病毒BHRE1基因抗辐射作用的研究 |
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| 引用本文： | 黄海,潘星华.EB病毒BHRE1基因抗辐射作用的研究[J].临床耳鼻咽喉头颈外科杂志,1998,12(3):131-133. |
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| 作者姓名： | 黄海 潘星华 |
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| 作者单位： | [1]第二军医大学附属长海医院工程国家重点实验室 [2]复旦大学遗传学研究所基础工程国家重点实 |
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| 摘 要： | 为了解ＥＢ病毒编码基因ＢＨＲＦ１的表达对鼻咽癌细胞抵抗放射线损伤能力的改变，构建携有ＢＨＲＦ１的高表达载体，并转染鼻咽癌细胞株ＣＮＥ２细胞，观察转梁前后癌细胞在放射线照射后于裸鼠体内成瘤能力改变的情况。结果发现，在放射线照射前，ＢＨＲＦ１表达细胞在裸鼠体内形成肿瘤时间较晚，肿瘤重量较轻（Ｐ〈０．０１）；而经放射线照射后，ＢＨＲＦ１表达细胞在裸鼠体内的成瘤能力于对照组（Ｐ〈０．０１），经原位末端标记
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| 关 键 词： | EB病毒 BHRF1基因 鼻咽肿瘤 放射疗法 抗辐射 |
The study of radioresistance of BHRF1 gene] |
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| H Huang,X Pan,X Kong.The study of radioresistance of BHRF1 gene][J].Journal of Clinical Otorhinolaryngology,1998,12(3):131-133. |
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| Authors: | H Huang X Pan X Kong |
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| Institution: | Changhai Hospital, Second Military Medical University, Shanghai 200433. |
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| Abstract: | In order to learn relationship between the expression of BHRF1 gene and radiative sensitivity of cells of nasopharyngeal carcinoma. High BHRF1 expressive vector was set up and transfected in the cell line of nasopharyngeal carcinoma, CNE2. Then the cells were radiated to test its ability of clone formation in nude mice. The results showed that the tumors formed by BHRF1-CNE2 cells were lighter before radiation and weighter after radiation (P < 0.01), and apoptotic index of BHRF1-CHE2 was less than control groups (P < 0.01). So, it suggests that the BHRF1 expression could prevent apoptosis by enhancing the resistibility of the tumor cells to radiation. |
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