| 食源性肥胖大鼠弓状核神经肽Y和GnRH的变化及对卵泡发育的影响 |
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| 引用本文： | 赵方欣,张洪芹.食源性肥胖大鼠弓状核神经肽Y和GnRH的变化及对卵泡发育的影响[J].滨州医学院学报,2011,34(3):175-178. |
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| 作者姓名： | 赵方欣 张洪芹 |
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| 作者单位： | 烟台开发区医院,烟台市,264003;滨州医学院组织学与胚胎学教研室 |
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| 摘 要： | 目的观察食源性肥胖大鼠弓状核神经肽Y和促性腺激素释放激素（GnRH）的变化及对卵泡发育的影响,为肥胖所致不孕提供实验依据。方法刚断乳SD雌性大鼠,饲以自制高能饲料,喂养14周后,根据Lee’s指数及连续阴道涂片,筛选出肥胖不孕模型;另有饲普通饲料组为对照组。观察神经肽Y（NPY）、瘦素受体（ob-R）及促性腺激素释放激素（GnRH）在其下丘脑弓状核的表达情况,并测定血清雌二醇（E2）、睾酮（T）、瘦素（leptin）、卵泡刺激素（FSH）、黄体生成素（LH）的水平。结果肥胖不孕大鼠血清E2、T、leptin水平较对照组明显升高（P（0.05）,FSH、LH水平较对照组明显降低（P（0.05）;下丘脑弓状核NPY表达较对照组增强（P（0.05）;ob-R及GnRH表达较对照组均减弱（P（0.05）。结论食源性肥胖大鼠由于神经内分泌-代谢失调引起NPY表达增强GnRH水平降低,导致下丘脑-垂体-卵巢轴功能失调性无排卵引起不孕。
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| 关 键 词： | 神经肽Y 促性腺激素释放激素 肥胖 不孕 大鼠 |
The changes of NPY and GnRH in arcuate nuclcus and the effect of NPY and GnRH on the folliole of the diet-induced obese rats |
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| ZHAO Fangxin,ZHANG Hongqin.The changes of NPY and GnRH in arcuate nuclcus and the effect of NPY and GnRH on the folliole of the diet-induced obese rats[J].Journal of Binzhou Medical College,2011,34(3):175-178. |
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| Authors: | ZHAO Fangxin ZHANG Hongqin |
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| Institution: | ZHAO Fangxin1 ZHANG Hongqin21 Yantai Kaifaqu Hospital,Yantai 264003,2 Department of Histology and Embryology,Binzhou Medical University |
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| Abstract: | Objective To investigate the changes of ncuropeptide Y(NPY)and GnRH in arcuate nucleu(ARC) and the effect of NPY and GnRH on the folliole of the diet-induced obese rats,and to assess the role of NPY and GnRH in the rat of diet-induced obesity.Methods To establish obese-infertile animal models by feeding the animals with high fat diet for 14 weeks.Expression patterns of NPY,OB-R and GnRH in arcuate nucleus of hypothalamic were observed and the levels of serum E2,T,leptin,FSH and LH in sera were evaluated.Res... |
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| Keywords: | neuropeptide Y(NPY) gonadotropin-releasing hormone obesity infertility rats |
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