The Ca^2＋ Antagon i z i ng Effect of Oh i nese Cobra Venom Factor on Formation of Macrophage-derived Foam Cells

Purpose CCVF was isolated from Chinese cobra （Naja naja） venom, its Ca^2＋ antagonizing effect on formation of macrophage-derived foam cells was explored in these studies. Methods Foam cell models were induced with C57BL/6J mouse peritoneal macrophages incubated in 10mg/L oxidized low density lipoprotein （OLDL）, and their intracellular Ca^2＋ levels influenced both slowly and transiently by CCVF were determined with the technique of Ca^2＋ fluorescent indicator. Results The intracellular Ca^2＋ level with the macrophages incubated in 10mg/L OLDL and lOmg/L CCVF was 40.2%of the macrophages incubated in 10mg/L OLDL （P〈0.05）; While the transient influence of CCVF on the intracellular Ca^2＋ levels were not significant. Conclusion CCVF exerted a long-lasting antagonizing role on the enhancement of intracellular Ca^2＋ levels, thus inhibited the formation of macrophage-derived foam cell.