聚合酶链反应在小儿支原体肺炎诊断中的应用价值

为探讨ＰＣＲ在小儿支原体肺炎诊断中的实际应用价值，对４７例临床疑诊支原体肺炎的病儿做了咽拭子ＰＣＲ－ＭＰ测定，同时做了血冷凝集试验（ＣＡＴ）。结果：ＰＣＲ－ＭＰ阳性２１例，真阳性１５例，假阴性１例，敏感度为９３．８％。假阳性６例，占检测标本的１２．８％，占检测报告阳性总数的２８．６％，ＣＡＴ阳性１２例。确诊支原体肺炎１６例，其中１５例经ＰＣＲ早期确诊。结论：ＰＣＲ－ＭＰ测定有利于支原体肺炎的早期诊

Evaluation of Polymerase Chain Reaction in Diagnosis of Mycoplasmal Pneumonia in Children

To evaluate the applicability of polymerase chain reaction (PCR) in diagnosis ofmycoplasmal pneumonia in children, 47 specimens of pharyngal swab from suspected cases werecollected. PCR was used to detect the mycoplasma pneumonia (MP) and cold agglutinin test(CAT) was simultanlously employed. The results showed that 21 in 47 cases were positive forMP, of them, 15 were true - positive, 6 were false - positive, one case was false - negtive. The sensitivity was 93. 8 %. 12 in 47 cases were positive for CAT. 16 cases were confirmly diagnosed asmycoplasmal pneumonia, of them, 15 were diagnosed early by means of PCR. PCR is useful .forearly diagnosis of mycoplasmal pneumonia in children. Since there are some false- positive and false- negtive, it has to be used in combineation with clinic feature.