Isolation of follicular dendritic cells from human tonsils and adenoids. II. Immunocytochemical characterization |
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Authors: | Ernst Heinen,Chantal Lilet-Leclercq,David Y. Mason,Harald Stein,Jacques Boniver,Daniel Radoux,C cile Kinet-Denoë l,L on J. Simar |
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Affiliation: | Ernst Heinen,Chantal Lilet-Leclercq,David Y. Mason,Harald Stein,Jacques Boniver,Daniel Radoux,Cécile Kinet-Denoël,Léon J. Simar |
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Abstract: | Follicular dendritic cells (FDC) are specialized cells found only within lymphoid follicles. They bind immune complexes and play a role in the presentation of antigen to follicular B cells and in the generation of B cell memory. In the present report the isolation of FDC from human tonsils and adenoids is described. These isolated cells have an unusual spherical arrangement and enclose lymphocytes within extensions of their membranes. Their ultrastructural features are similar to those observed in situ. The reactivity of isolated FDC with a number of monoclonal antibodies was analyzed by immunofluorescence and by immunostaining (at the electron microscopic level) with colloidal gold. In keeping with the results of previous investigations on tissue sections IgM, IgG and IgA (but not IgD) can be detected on the surface of isolated FDC, as can C3b receptors and the FDC-associated antigen detected by monoclonal antibody R4/23. The immunoglobulins associated with FDC are mostly embedded in an electron-dense material. The majority of the lymphoid cells enclosed within the membrane extensions of FDC are of B cell type. These results suggest that isolated FDC may be suitable for further in vitro investigation of their role in the humoral immune response. |
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