复方降压1号对两肾一夹大鼠的降压作用及其毒性

目的:研究复方降压1号口服给药对两肾一夹大鼠的降压作用及毒性。方法:采用两肾一夹(2K1C)法复制肾血管性高血压大鼠模型,取32只造模成功的大鼠随机分为复方降压1号高低剂量(0.6g/kg、0.2g/kg)组、尼群地平(0.001g/kg)组和模型对照组(蒸馏水5ml/kg),另设假手术组(蒸馏水5ml/kg)。按上述设定剂量每日灌胃给药1次,连续4周,每周测量血压和体重,4周后测定血脂、右肾湿重、肾功能和尿酸含量。采用分组法测定小鼠口服半数致死量(LD50)。结果:复方降压1号高低剂量组和尼群地平阳性组大鼠血压明显降低(P<0.05或P<0.01),其高剂量组降压作用较尼群地平组强(P<0.05),而对血脂、肾功能、尿酸和右肾湿重及其系数无明显影响。小鼠灌胃给药的LD50为18.066g/kg,按体重和动物系数计算约为成人量的643倍和72倍。结论:复方降压1号能明显降低两肾一夹大鼠的血压,其高剂量组降压效果强于尼群地平,对血脂、肾功能和尿酸无明显影响;其小鼠口服毒性较小。

Antihypertensive effect of Fufang-jiangya-I in the 2K1C rats and acute toxicity

Objective： To observe the antihyperleusive effect of Fafang- jiangya-1（ FFJY1 ） in the 2K1C rats and the acute toxicity of this drag by oral administration. Methods ： Rats of renovaseular hypertension were induced by two-kidney, one-clip method （2K1C）. Thirty-two prepared rat models were randomized into groups of high or low dose of FFJY1 （0.6 g/ kg,0. 2 g/kg） , nitrendipine（0. 001 g/kg） , animal controls （ distilled water,5 ml/kg） and sham-operation （distilled water,5 ml/kg）. The rats were intragastrieally administered once a day for four weeks by the given dose described above, with weekly measurement of the blood pressure and body weight. Four weeks later, the experimental animals were examined in blood fat,humid weight of right kidney,renal function and concentration of uric acid. LD50 p. o for the mice was measured in subgroups. Results：The blood pressure in high dose of FFJYI （0.6 g/kg） and nitrendipine （0.001 g/kg） decreased significantly （ P 〈 0.05 or P 〈 0. 01 ）. Antihypertensive effeet in the rats with high-dose was stronger than that of nitrendipine （P 〈 0. 05 ） , but there was no significant effect concerning the level of blood fat, index on renal function and blood uric acid. LD50 of FFJY1 p. o was 18. 066 g/kg which was approximately 643 times and 72 times compared with the body weight of human beings and estimation of animal coefficient. Conclusion： FFJY1 can significantly decrease blood pressure in the 2K1C rats and the antihypertensive effect with high-dose seems stronger than that of nitrendipine. The drug produces no obvious effect on blood fat, renal function and uric acid, but it has lower toxicity in mice by oral FFJY1.