不同降压药物对自发性高血压大鼠模型左室心肌细胞中TRPC3及TRPC6表达的影响

目的:探讨缬沙坦、雷米普利及氨氯地平对自发性高血压大鼠（SHR）左室心肌中瞬时受体通道蛋白C亚族3及6(TRPC3及TRPC6)表达的影响。方法: 将24只12周龄SHR大鼠随机分为4组，即SHR组、缬沙坦组、雷米普利组及氨氯地平组，每组6只。另以6只同龄的Wistar Kyoto大鼠(WKY)为正常对照组。给药4周后，检测各组大鼠的血压、左室质量指数、左室心肌细胞横径；RT-PCR及Western Blot检测TRPC3及TRPC6 mRNA 及其蛋白的表达。结果: SHR组血压、左室质量指数及左室心肌细胞横径均明显高于对照组（P<0.05），3个药物组上述指标均较SHR组降低（P<0.05）；5个组均有TRPC3及TRPC6的表达，SHR组TRPC3及TRPC6 mRNA及其蛋白的表达显著高于对照组（P<0.05），3个药物组TRPC3 mRNA及TRPC6 mRNA及其蛋白的表达均显著低于SHR组（P<0.05），缬沙坦组TRPC3 mRNA及其蛋白表达的减少最显著（P<0.05）；3个药物组TRPC6 mRNA及其蛋白的表达有所下降，但组间比较差异无显著性。结论: SHR组及对照组大鼠均有TRPC3及TRPC6的表达，TRPC3及TRPC6可能共同参与调节心肌肥厚的病理生理过程；缬沙坦可能通过抑制TRPC3蛋白的表达参与逆转左室肥厚的过程。

Effects of antihypertensive drugs on expression of TRPC3 and TRPC6 in left ventricle of spontaneously hypertensive rats

AIM: To investigate the effect of valsartan,ramipril and anlodipine on the expression of TRPC3 and TRPC6 in the left ventricle in spontaneously hypertensive rats(SHR).METHODS: Twelve-week-old SHRs were randomly divided into four groups: valsartan-treated group(Val),ramipril-treated group(Ram),amlodipine-treated group(Aml) and untreated group(SHR).Twelve-week-old Wistar-Kyoto(WKY) rats were used as control group.After 4 weeks of drug intervention,blood pressure(BP),left ventricular mass index(LVMI),left vent...