Exendin-4对脂肪细胞分化及糖脂代谢相关基因mRNA表达的影响 |
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引用本文: | 肖新华,赵向,曹仁贤,文格波,刘江华. Exendin-4对脂肪细胞分化及糖脂代谢相关基因mRNA表达的影响[J]. 中华糖尿病杂志, 2011, 0(7): 489-492 |
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作者姓名: | 肖新华 赵向 曹仁贤 文格波 刘江华 |
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作者单位: | 南华大学第一附属医院内分泌科,衡阳421001 |
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基金项目: | 国家自然科学基金资助项目(81070667);湖南省教育厅青年基金(108092) |
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摘 要: | 目的探讨Exendin-4对3T3-L1前脂肪细胞的分化及糖脂代谢相关基因mRNA表达的影响。方法体外培养3T3-L1前脂肪细胞,在脂肪细胞分化成熟过程中的不同时期分别用Exendin-4等干预,采用油红O染色,观察脂肪细胞分化及脂质积聚情况;采用荧光定量PCR检测脂肪细胞糖脂代谢标志基因GLUT-4、PPARγ、HSLmRNA表达水平。酶法测定脂肪细胞的甘油三酯含量。结果分化成熟的脂肪细胞经油红O染色可见细胞质内大片脂滴呈亮红色,而未分化细胞不被油红O染色。在脂肪细胞分化第0天和第6天用Exendin-4干预,脂肪细胞内TG的含量较空白组增加(P〈0.01),GLUT-4、HSL、PPARγ mRNA的表达上调(P〈0.01);在脂肪细胞分化的第12天干预,Exendin-4对肪细胞分化及相关基因mRNA的表达与空白组无明显差异。结论Exendin-4促进脂肪细胞的分化并上调糖脂代谢相关基因GLUT-4、PPARγ、HSL mRNA表达,可能为Exendin-4抗糖尿病的部分作用机制。
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关 键 词: | Exendin-4 甘油三酯 分化 |
Effects of exendin-4 treatment on differentiation of 3T3-L1 preadipocyte and on mRNA expressions of glucose and lipid metabolic marker genes |
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Affiliation: | XIAO Xin-hua , ZHAO Xiang , CAO Ren-xian , et al.( Department of Endocrinology, The First Affiliated Hospital of Nanhua University, Hengyang 421001, China) |
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Abstract: | Objective To investigate the effect of exendin-4 on differentiation of 3T3-L1 preadipocytes and mRNA expression of glucose and lipid metabolic marker genes. Methods 3T3-L1 preadipocytes were cultured in vitro and diferentiated into the matured adipocytes. Cell diferentiation and lipid accumulation were determined by oil red O staining. Glucose and lipid metabolism marker genes (GluT-4, PPAR-γ and HSL) mRNA expression were evaluated by quantitative real-time PCR. The triglyceride contents of isopropanol extracts prepared from 3T3-L1 preadipocytes were quantitated with an acetylacetone-based colorimetrie kit. Results Mature adipocytes showed large lipid droplets within cytoplasm stained by oil red O. Preadipocyte differentiation were promoted, TG content and the mRNA expressions of GluT-4, HSL and PPAR-γ were increased (P%0.01) when 3T3-L1 preadipocytes were treated with exendin-4 at day 0 and day 6. Compared with control group, the effects of exendin 4 on adipocytes differentiation and mRNA expression of these genes had no significant difference when 3T3-L1 preadipocytes were treated at day 12. Conclusion Exendin-4 promotes the preadipocytes differentiation, increases glucose and lipid metabolism marker genes mRNA expression, which may be some new mechanisms of exendin-4 anti-diabetes action. |
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Keywords: | Exendin-4 Triglyceride Differentiation |
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