Localization of urokinase-type plasminogen activator mRNA in the adult mouse brain |
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| Affiliation: | 1. Department of Veterinary Sciences, University of Wyoming, Laramie, WY 82070, USA;2. Neuroscience Graduate Program, University of Wyoming, Laramie, WY 82070, USA;3. Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria 3010, Australia;4. MassGeneral Institute for Neurodegenerative Disease, Charlestown, MA 02129, USA;1. LaNCE, Department of Neuroscience, University of the Basque Country (UPV/EHU), Leioa, Spain;2. Department of Neurology, The Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States;3. Neuropharmacology Group, University of the Basque Country (UPV-EHU), Leioa, Spain |
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| Abstract: | Urokinase-type plasminogen activator (uPA) is an inducible serine protease, secreted by a variety of cell types, that functions in fibrinolysis and has been implicated also in events such as cell migration and tissue remodeling and repair. To explore the role of uPA in the adult brain, we have now screened the whole mouse brain for cells expressing the uPA gene through in situ hybridization using 35S-complementary RNA. uPA mRNA was visualized predominantly in three regions: (1) the subicular complex, (2) the entorhinal cortex, (3) the parietal cortex, where the signal was somewhat lower and confined to layers IV and VI. Weaker signals were seen in the basolateral nucleus of the amygdala and in the anterodorsal thalamic nucleus, and also in the hilus of the dentate gyrus where labeling was slightly over background. Cells exhibiting uPA mRNA signaling were large neurons according to morphological criteria. These results support the view of uPA being involved in neuronal functions of the adult brain, specifically in the hippocampal formation and the parietal cortex. |
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