Antioxidant and anti-inflammatory activity of Polygonatum sibiricum rhizome extracts |
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Authors: | Trishna Debnath Sa Ra Park Da Hey Kim Jeong Eun Jo Beong Ou Lim |
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Affiliation: | College of Biomedical & Health Science, Department of Life Science, Konkuk University, Chungju 380-701, South Korea;Department of Food Science and Technology, and Functional Food Research Center, College of Agriculture an Life Science, Chonnam National University, Gwangju 500-757, Korea., E-mail: |
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Abstract: | ObjectiveTo investigate the antioxidant and anti-inflammatory activities of distilled water extract of fresh Polygonatum sibiricum (P. sibiricum) rhizome.Methods: The extracts were tested for antioxidant activity by using DPPH (1,1-diphenyl-2-picryl-hydrazyl), and hydroxyl (OH•) radicals scavenging activity. The level of intracellular reactive oxygen species (ROS) was determined in H2O2 treated chang liver cells. Anti-inflammatory activity was evaluated by the inhibition of nitric oxide (NO), inducible nitric oxide synthase (iNOS), as well as tumor necrosis factor-alpha (TNF-α) protein expression in a lipopolysaccharide stimulated Raw 264.7 murine macrophages cell line.Results: P. sibiricum water extracts scavenged DPPH, OH• radicals and decreased the level ROS. The extracts reduced NO reduction and inhibited the expression of iNOS and TNF-α proteins.Conclusions: The findings indicate that water extracts of P. sibiricum could be considered as natural antioxidants and anti-inflammatory agents for food and drug industries. |
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Keywords: | Reactive oxygen species Phenolic content Lipopolysaccharide iNOS |
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