补充VA、VE对梭曼中毒大鼠体内抗氧化能力影响的初探

目的 研究梭曼对急性中毒大鼠的自由基损伤作用，探讨维生素A、E的抗氧化作用。方法 雄性大鼠，随机分为对照组、阳性组、VA组、VE组。灌胃10d，第11d除阴性组外其余各组大鼠均皮下注射0．9LD50梭曼，2h后取样，测定各组全血和肝组织的谷胱甘肽过氧化物酶（GSH－Px)、过氧化氢酶（CAT）、VE和总抗氧化力（T－AOC）。结果 梭曼中毒2h后，GSH－Px活性、T－AOC水平、VE含量降低。维生素A、E均有效的抑制其变化。结论 梭曼对大鼠有自由基损伤作用，维生素A、E对梭曼中毒大鼠过氧化损伤具有保护作用，提示维生素A、E对梭曼中毒有预防作用。

Effect of VA and VE supplementation on antioxidation injuried by Sonan poisining in rat

Aim To study the effects of free radic al injury induced by soman.Discuss effect of antioxidat ion of V A and V E against free radical injury induced by Soman.Glutathion peroxidate(GSH -Px)and catalase(CAT)activity of blood and liver in rat inj ected by Soman.Methods male rats were randomly divided into4groups ,negative group,positive group,V A group and V E group.After 10d,rats in other groups are given0.9LD50Soman from hypodemic inject ion except negative group.After2h,rats were killed and the samples were collected.The blood and liver GSH -Px ,CAT,V E and T -AOC(total antioxidative capacity)were determined.Results GSH -Px activity,V E contents and T -AOC level of toxicant rat injected by Soman dec reased after 2hour.V A and V E in-hibated the change effectevely.Conclusion There is a mechanism of free radical injury by soman and signific antly antioxidative effect of V A and V E against free radical injury induced by Soman in rat.It is suggested that V A and V E have protective effect against free radical injury induced by Soman in rat.