Tip30基因修饰的ACC-M细胞裸鼠体内致瘤性观察

目的:观察肿瘤抑制基因Tip30修饰后的人涎腺腺样囊性癌高转移细胞(ACC鄄M)所致裸鼠移植瘤体内生长的变化。方法:BALB/Cnu/nu鼠30只(4周龄,体重18￣20g),随机分3组,各10只;Tip30基因修饰的ACC鄄M细胞(AdTIP30/ACC鄄M)、野生型ACC鄄M细胞(wt/ACC鄄M)及对照基因LacZ修饰的ACC鄄M细胞(AdLacZ/ACC鄄M)分别注射小鼠颌下区皮下,观察肿瘤生长情况及小鼠存活时间。结果:(1)经TIP30基因修饰组肿瘤出现的时间比野生型对照组及基因对照组晚3d,且肿瘤生长缓慢(P<0.01);(2)存活时间显示,基因修饰组(53.0±3.3)d,有2只长期存活;野生型对照组(26±2.2)d;对照基因组(29±2.4)d。结论:抑癌基因Tip30能够显著抑制人涎腺腺样囊性癌细胞在裸鼠体内的生长,延长荷瘤小鼠存活期。

Investigation of the Tumorigenicity of Tumor Suppressor Gene Tip30 Modified ACC-M Cells in BALB/C Mice

Objective: To observe the changes of the tumor suppressor gene Tip30 on the growth of transplant tumor on BALB/C mice with high metastasis cells of human salivary adenoid cystic carcinoma. Methods: 30 BALB/C nude mice(four-week-old, weighted 18-20g), divided randomly into 3 groups with 10 in each group, were injected at submandibular with AdTip30/ACC-M , wt/ACC-M and AdLacZ/ ACC-M . Observed the tumor growth and survival period. Results: The results of AdTip30/ACC-M showed that the tumor occurs 3 days later than that of wt/ACC-M and AdLacZ/ACC-M, and the tumor grew much slower(P<0.01); the survival period of AdTip30/ACC-M was (53.0±3.3)d , with two mice survives for good, while wt/ACC-M was (26±2.2)d and AdLacZ/ACC-M was (29±2.4)d. Conclusion: Tumor suppressor gene Tip30 could significantly inhibit the growth of human SACC cells in nude mice and prolong survival time.