人参总皂甙对正常人和再生障碍性贫血患者造血祖细胞的刺激增殖作用

为了解人参对造血祖细胞的作用,应用红系祖细胞(BFU-E、CFU-E)和粒单系祖细胞(CFU-GM)体外培养技术,观察人参总皂甙(TSPG)对正常人和29例再生障碍性贫血(再障)患者的祖细胞刺激增殖作用,并与甲基睾丸素(甲睾)相比较。TSPG在20μg/ml浓度时,可使正常BFU-E、CFU-E和CFU-GM产率分别提高37.8±2.9%、31.4±2.9%和33.4±4.0%。去除红细胞生成素,TSPG亦具有直接刺激红系祖细胞增殖作用,而甲睾对CFM-GM无作用。29例再障中雄激素反应型14例(48.1%),均同时呈现对人参的敏感性(集落数增加30%以上)。而免疫介导型及干细胞缺少型中人参的作用均不能表达。提示TSPG的作用机制可能是通过增强红细胞生成素等造血生长因子的活性间接地促进骨髓造血。

Effect of Total Saponins of Panax Ginseng on Hematopoietic Progenitor Cells in Normal Human and Aplastic Anemia Patients

Ginseng was said to be benefit for anemia in TCM. Proliferation effects of total saponins of panax ginseng (TSPG) on hematopoietic progenitor cell in normal individuals and 29 patients with aplastic anemia (AA) were observed by bone marrow culture of BFU-E, CFU-E, CFU-GM in vitro compared with methyltestosterone (MT). The results showed that TSPG might prompt proliferation of normal progenitor cells at the concentration of 20 micrograms/ml. The number of BFU-E, CFU-E and CFU-GM had increased by 37.8 +/- 2.9%, 31.4 +/- 2.9% and 33.3 +/- 4.0% over the controls respectively; furthermore TSPG was still useful to BFU-E, CFU-E growth without Epo in vitro, although the colony numbers were very lower. Otherwise MT was useless to CFU-GM. 14 of the 29 patients with AA who responded to MT showed sensitivity to TSPG in marrow culture (the rising rate of colony formation exceeded 30%), but immune-mediated AA (patient's PBMNC suppressed normal hematopoiesis) and stem cell-decreased AA (few of colony was formed) showed almost no expression for TSPG activity because of immunological suppression system and absence of progenitors.