增生性瘢痕和瘢痕疙瘩P53基因多态性的分析

目的 探讨p53基因codon-72部位的多态性分布是否与增生性瘢痕及瘢痕疙瘩的易发性有关。方法 以日本医科大学整形外科行手术治疗患者的切除瘢痕标本为材料，利用限制性片段长度多态性分析法(RFLP)及DNA序列分析法(DNA-sequence)，对54例瘢痕疙瘩标本和30例增生性瘢痕标本的p53基因codon-72部位编码精氨酸(Arg)和脯氨酸(Pro)的等位基因CGC(Arg)和CCC(Pro)进行了分析。结果 与正常日本人群外周血p53基因codon-72部位的多态性频度分布相比，瘢痕疙瘩差异无显著性，而增生性瘢痕则差异有显著性。此外，由穿耳孔所致的耳部瘢痕疙瘩CGC(Arg)基因型显著增高。结论 推测p53codon 72部位具有CGC／CGC(Arg／Arg)纯合型者耳部瘢痕疙瘩的发生率明显增高，具有CCC／CCC(pro／pro)纯合型者增生性瘢痕发生率增高。

Investigation of p53 polymorphism for genetic predisposition of keloid and hypertrophic scar

Objective To investigate the codon |72 polymorphism of the tumor suppressor gene p53,codon |72 encodes arginine(Arg)or proline(Pro)for a genetic predisposition to keloid or hypertrophic scar. Methods The distribution of codon 72 polymorphism of p53 gene was analyzed from the 54 keloid and 30 hypertrophic scar(HS)of the Japanese patients with restriction fragment length polymorphism analysis and DNA sequence analysis. Results The frequency of the Proline |encoding alleles and Arginine |encoding alleles in the hypertrophic scar patients and the piercing |induced ear |lobe keloid patients,was deviated significantly from that in the normal Japanese controls. Conclusions The Proline |encoding allele and Arginine |encoding allele could have the risks for the hypertrophic scar and the piecing |induced ear |lobe keloid.Also,the pathogenesis of the hypertrophic scar seems to be different from that of keloid at the molecular level. ;