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| 从抗-HEV阴性的戊型肝炎病人血清中检测到1株HEV变异株 | |
| 引用本文： | 李奎,庄辉,王佑春,朱万孚,唐荣兰,闭迎新,韦启援,疗丹,郭堑,韦福邦,黄运泰. 从抗-HEV阴性的戊型肝炎病人血清中检测到1株HEV变异株[J]. 中华微生物学和免疫学杂志, 2000, 20(2): 164-166 |
| 作者姓名： | 李奎 庄辉 王佑春 朱万孚 唐荣兰 闭迎新 韦启援 疗丹 郭堑 韦福邦 黄运泰 |
| 作者单位： | 1. 北京医科大学微生物学系 2. 中国药品生物制品检定所 3. 广西柳州卫生学校 4. 柳州市工人医院 |
| 基金项目： | “九五”国家科技攻关计划资助项目!(969060307) |
| 摘 要： | |
| 关 键 词： | 戊型肝炎 核苷酸序列 氨基酸序列 HEV变异株 |
| 修稿时间： | 1998-10-19 |
A variant hepatitis E isolated from a hepatitis E patient with negative anti-HEV |
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| LI Kui ,ZHUANG Hui,WANG Youchun,et al.. A variant hepatitis E isolated from a hepatitis E patient with negative anti-HEV[J]. Chinese Journal of Microbiology and Immunology, 2000, 20(2): 164-166 | |
| Authors: | LI Kui ZHUANG Hui WANG Youchun et al. |
| Affiliation: | LI Kui *,ZHUANG Hui,WANG Youchun,et al. *Department of Microbiology,Beijing Medical University,Beijing 100083,P.R.China Corresponding author:ZHUANG Hui,E mail:zhuanghui @ publica.bj.cninfo.net |
| Abstract: | Objective To investigate genotypes of hepatitis E viruses isolated from hepatitis E patients with negative anti HEV. Methods Acute phase sera of 22 hepatitis patients with negative anti HAV IgM, HBsAg , anti HBc IgM, anti HCV and anti HEV were detected for HEV RNA using a RT nPCR based on a set of degenerate primers derived from open reading frame 2 of HEV. The positive PCR products were directly sequenced. Results Among 22 acute hepatitis patients, 9(40.9%) were HEV RNA positive. Six PCR products of 9 sera were directly sequenced. Five of them shared the same subtype with HEV Chinese strain (L25595), with the nucleotide identity of 98.0% and the amino acid identity of 99.0%. The remainder, designated HEV LZ 105, was a variant of HEV. The nucleotide and amino acid homology of HEV LZ 105 with HEV(China),HEV(Burma),HEV(Mexico),and the newly identified HEV US 1 and swine HEV was 77.4%,78.1%,74.3%,77.4%, 77.1% and 91.0%,91.0%,89.0%,93.0%,93.0%,respectively. Conclusion A variant of HEV was isolated from a hepatitis E patient with negative anti HEV. |
| Keywords: | Hepatitis E virus Nucleotide sequences Amino acid sequences |
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