| 人类精液冷冻前后精子运动变化的计算机辅助分析 |
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| 引用本文： | 刘美意,邓顺美,马春杰,陈爱平,姜彦嘉,文任乾,王奇玲,唐立新,黄健初,姚晓涛. 人类精液冷冻前后精子运动变化的计算机辅助分析[J]. 中华男科学杂志, 2004, 10(6): 431-433 |
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| 作者姓名： | 刘美意 邓顺美 马春杰 陈爱平 姜彦嘉 文任乾 王奇玲 唐立新 黄健初 姚晓涛 |
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| 作者单位： | 广东省计划生育专科医院人类精子库,广东,广州,510600 |
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| 摘 要： | 目的 :利用计算机辅助精液分析 (CASA)系统分析冷冻前后人类活动精子的运动参数 ,并观察相关参数的变化规律。 方法 :2 38份精液样本在冷冻前和复苏后均采用CASA分析其运动参数。 结果 :复苏后精子活动率下降 ;除精子鞭打频率 (BCF)外 ,冷冻前与复苏后全部精子运动参数呈显著正相关且差异存在显著性 ;平均路径速度 (VAP)、直线速度 (VSL)、曲线速度 (VCL)下降 ,精子头侧摆幅 (ALH)下降 ,但直线性 (LIN)、前向性 (STR)升高。 结论 :CASA系统可对冻融前后精子运动参数进行客观细致的分析 ,有一定的临床应用价值。
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| 关 键 词： | 精子 运动参数 计算机辅助精液分析系统 冷冻保存 人类 |
| 文章编号： | 1009-3591(2004)06-0431-03 |
| 修稿时间： | 2003-10-15 |
Comparison of Sperm Motion Parameters in Pre-freeze and Post-thaw Semen Samples Using Computer-Assisted Sperm Analysis |
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| Liu Meiyi,Deng Shunmei,Ma Chunjie,Chen Aiping,Jiang Yanjia,Wen Renqian,Wang Qiling,Tang Lixin,Huang Jianchu,Yao Xiaotao. Comparison of Sperm Motion Parameters in Pre-freeze and Post-thaw Semen Samples Using Computer-Assisted Sperm Analysis[J]. National journal of andrology, 2004, 10(6): 431-433 |
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| Authors: | Liu Meiyi Deng Shunmei Ma Chunjie Chen Aiping Jiang Yanjia Wen Renqian Wang Qiling Tang Lixin Huang Jianchu Yao Xiaotao |
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| Affiliation: | Human Semen Bank, Guangdong Family Planning Hospital, Guangzhou, Guangdong 510600, China. dxlmyi@pub.guangzhou.gd.cn |
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| Abstract: | Objective: To observe sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis(CASA) system. Methods: Semen analyses of 238 samples before freezing and after thawing were separately performed by Hamilton-Thorne Sperm Analyzer. Results: Sperm motility in post-thaw samples was significantly decreased. There was significant correlation and difference between pre-freeze and post-thaw samples in sperm motion parameters,including average path velocity (VAP),straight line velocity (VSL),curvilinear velocity (VCL),amplitude of lateral head displacement (ALH),straightness (STR) and linearity (LIN),except beat cross frequency (BCF). The percentage of sperm movement velocity parameters (VAP,VSL and VCL) and moving pattern parameters (ALH) significantly decreased,while that of LIN and STR significantly increased in post-thaw samples. Conclusion: CASA system is of clinically applied value and is a useful tool for evaluating sperm motion parameters in pre-freeze and post-thaw semen samples. |
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| Keywords: | sperm motion parameter computer-assisted sperm analysis system cryopreservation human |
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