川芎嗪对复氧后损伤心肌细胞的影响

目的：观察川芎嗪对大鼠心室肌细胞缺氧－复氧损伤的作用，方法：在缺氧－复氧条件下，测量对照组和不同浓度川芎嗪组对杆形心肌细胞百分比，心肌细胞释放乳酸脱氢酶（LDH）和心肌细胞类脂过氧化产物－丙二醛（MDA）含量。结果：缺氧－复氧对大鼠心室肌细胞有损伤作用。浓度为4umol/L的川芎开始对缺氧－复氧损伤细胞有保护作用，它能抑制损伤细胞挛缩，提高损伤细胞生存率，抑制细胞LDH的释放，减少MDA的生成，且剂量越大，保护作用越好，即川芎嗪对缺氧－复氧损伤细胞的保护呈剂量依赖性，结论：川芎嗪对缺氧一复氧损伤的心肌细胞具有保护作用。它能显著地对抗缺氧－复氧对心室肌细胞的损伤。

Effects of trtramethylpyrazine to reoxygenation damage of rat ventricular myocytes

OBJECTIVE:To observe the effect of trtramethylpyrazine to rat ventricular myocytes with the anoxia reoxygenation damage.METHOD:we investigated the myocytes viability,myocytes releasing Lactate dehydrogenase (LDH) and forming lipids perxidation products malondiaodehyde(MDA)with different trtrmethylpyrazine concentration groups and control group in anosia reoxygenation damage condition.RESULTS:Anoxia reoxygenation had damage to the rat ventricular myocytes.Trtramethylpyrazine inhibited the myocytes constriction,increased the cell viability and reduce the releasing of LDH from cells.Formation of MDA was reduced from 4umol/L and the more dose of trtramethylpyrazineuse use, the better salutary effects. lt suggest that trtramethylpyrazine reduced anoxia reoxygenation damage in a dose dependent manner.CONCLUSIONS Trtramethylpyrazine could protect anoxia reoxygenation damage of rat ventricular myocytes,and the treatment with trtramethylpyrazine has significant resistant to the anoxia reoxygenation damage.