Survivin、PCNA在乳腺癌中的表达及其意义

目的:探讨凋亡抑制蛋白(Survivin)、增殖细胞核抗原(Proliferatingcellnuclearantigen,PCNA)在乳腺癌中的表达及其临床意义。方法:用免疫组化染色检测74例乳腺癌组织中Survivin和PCNA蛋白的表达情况。结果:在乳腺癌组织中,Survivin蛋白的阳性表达率为64.86%;Survivin蛋白表达阳性的乳腺癌细胞PCNA的标记指数达61.17%,明显高于Survivin蛋白表达阴性者的51.26%(P<0.05);乳腺癌中Survivin、PCNA阳性高表达者腋窝淋巴结转移率明显高于低表达者(P<0.05),5年生存率明显低于低表达及阴性者(P<0.05)。乳腺癌中Survivin、PCNA阳性表达与临床分期密切相关(P<0.05),与组织学分期无明显相关(P>0.05)。结论:检测Survivin及PCNA蛋白在乳腺癌组织中的表达,有助于乳腺癌进展和预后的判断。

EXPRESSIONS OF SURVIVIN AND PCNA IN BREAST CARCINOMA AND THEIR CLINICAL SIGNIFICANCE

Objective: To investigate the expressions of Survivin and proliferating cell nuclear antigen (PCNA) in breast carcinoma and their clinical significance. Methods: The expressions of Survivin and PCNA were tested by immunohistochemical method in 74 cases of breast carcinoma. Result: In the breast carcinoma, the positive expression rate of Survivin was 64.86 %. Labeling indexes of PCNA in breast carcinoma tissues with positive expression of Survivin reached 61.17 % and was significantly higher than that with negative expression of Survivin (51.26 %)(P<0.05). The rate of lymph node metastasis in the group of strong positive co-expression of the two genes was much higher than that in low positive or negative expressions of survivin and PCNA(P<0.05), but the 5 year survival rate was much lower than that in low positive or negative expression of Survivin and PCNA(P<0.05). The expressions of Survivin and PCNA were closely correlated with clinical staging(P<0.05), but was no significant correlation with histological types(P>0.05). Conclusion: Testing expressions of Survivin and PCNA could be served as indictors for evaluating the metastasis and prognosis in breast cancer.