N乙基马来酰亚胺敏感因子NSF与食管鳞癌细胞分化有关

目的：克隆食管鳞癌细胞ＥＣ８７１２分化过程中受ＡＴＲＡ调控的靶基因。方法：采用ＡＰ－ＰＣＲ技术从食管鳞癌细胞ＥＣ８７１２中克隆受ＡＴＲＡ调控的差异表达基因片段，并测序及作同源分析，采用Ｎｏｒｔｈｅｒｎ杂交进行验证。结果：分离到在食管癌细胞分化过程中表达下调的一基因片段。该基因从ＡＴＲＡ处理一天开始表达被明显抑制 种抑制持续整个诱导过程。该片段被克隆于ｐＧＥＭ－Ｔ载体并测序。同源分析结果表明，该基因

Esophageal squamous carcinoma cell differentiation-related gene N-ethylmalimide sensitive factor (NSF)

Objective:To clone ATRA regulated gene in esophageal squamous cancer cell line EC8712 Methods:By using AP PCR technique, differentially expressed gene fragments (ESTs) were isolated from ATRA treated EC8712 Cloning, sequencing and homology analysis were carried out to characterize an isolated differentitally expressed gene fragment Results:The differentially expressed gene fragment was isolated, cloned and sequenced, the gene expression was inhibited during whole ATRA treatment period, from first day to 9th day Homology analysis revealed that the fragment derives from human N ethylmalimide sensitive factor (NSF) gene, which is involed in vesicle transportation, expresses in various tissues, and plays important roles in differentiation of cells Conclusion:The decrease in activity of cellular vesicle transportation plays important roles in cellular differentiation process Down regulation of NSF might contribute to decreased activity of vesicle transportation and differentiation of human cancer cell