肝硬化患者外周血T淋巴细胞亚群变迁与免疫功能状态

目的 了解肝硬化(LC)患者外周血T淋巴细胞亚群的变化规律,探讨患者T细胞免疫功能状态.方法 采用流式细胞术检测32例代偿期LC患者(CC组)、35例失代偿期LC患者(LCC组)和63例健康者(对照组)外周血T淋巴细胞亚群水平.结果 与对照组比较,CC组和LCC组总T细胞及其各亚群细胞的绝对含量均下降,T4/T8比值升高,双阴性T细胞百分含量下降(P＜0.05);CC组T4/T8比值较LCC组更高(P＜0.05).结论 LC患者存在明显的T细胞免疫功能紊乱,包括各亚群细胞数量的减少及T4细胞相对于T8细胞的功能亢进.随着病情加重,T4细胞的应答能力进一步减退.

Alteration of T-cell subsets in peripheral blood and related immunologic function in patients with liver cirrhosis

Objective To study the alteration regularity of peripheral blood T lymphocyte subsets in patients with liver cirrhosis and investigate their immune functional status.Methods Peripheral blood T lymphocyte subsets were detected by flow cytometry in 32 compensatory cirrhosis patients(CC group),35 lose-compensatory cirrhosis patients(LCC group) and 63 healthy subjects(control group).The results were analyzed statistically.Results Absolute quantity of total T cell,CD8+T cell and double negative T cell were lower,and CD4+/CD8+ ratio was higher in CC and LCC group than those in control group.Compared with LCC group,the CD4+/CD8+ ratio in CC group was relatively higher.Conclusion There might be with immune disorder in LC patients,including the decrease of total T cell and each subset as well as the relative hyperfunction of CD4+T cell to CD8+T cell.As the diseases condition turning to severer,the responsibility of CD4+T cell could be further weaker.