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| hsa-miR-100的生物信息学分析 | |
| 引用本文： | 付海龙,徐广峰,史春梅,季晨博,郭锡熔,赵亚萍. hsa-miR-100的生物信息学分析[J]. 国际检验医学杂志, 2012, 33(18): 2177-2180 |
| 作者姓名： | 付海龙 徐广峰 史春梅 季晨博 郭锡熔 赵亚萍 |
| 作者单位： | 1. 蚌埠医学院,安徽蚌埠,233030;中国人民解放军第82医院检验科,江苏淮安,223001 2. 中国人民解放军第82医院检验科,江苏淮安,223001;南京医科大学儿科研究所,江苏南京,210029 3. 南京医科大学儿科研究所,江苏南京,210029;南京医科大学附属南京妇幼保健院儿童保健科,江苏南京,210004 |
| 基金项目： | 江苏省自然科学基金资助项目，江苏省淮安市产学研合作促进计划专项资金资助项目 |
| 摘 要： | 目的 对hsa-miR-100进行靶基因、功能预测等生物信息学分析,为深入研究hsa-miR-100功能提供理论和试验基础.方法 利用Pubmed检索miRNA-100相关文章,通过miRBase、NCBI、UCSC Browser等在线工具分析hsa-miR-100序列,应用miRanda、TargetScan及PicTar预测hsa-miR-100靶基因,结合已证实的靶基因进行功能富集分析和信号通路富集分析.结果 hsa-miR-100与多种肿瘤发生、发展有关,其序列在各物种间具有高度保守性.hsa-miR-100靶基因功能富集于基因沉默、染色质沉默、细胞生物合成负性调节等(P<0.01),涉及肿瘤信号通路、溶酶体信号通路、凋亡信号通路等信号转导通路(P<0.05).结论 hsa-miR-100可能参与肿瘤发生相关的生物学过程. |
| 关 键 词： | 微RNAs hsa-miR-100 生物信息学 靶基因 肿瘤 |
Bioinformatics analysis of hsa-miR-100 |
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| Fu Hailong , Xu Guangfeng , Shi Chunmei , Ji Chenbo , Guo Xirong , Zhao Yaping. Bioinformatics analysis of hsa-miR-100[J]. International Journal of Laboratory Medicine, 2012, 33(18): 2177-2180 | |
| Authors: | Fu Hailong Xu Guangfeng Shi Chunmei Ji Chenbo Guo Xirong Zhao Yaping |
| Affiliation: | 1,2(1.Bengbu Medical College,Bengbu,Anhui 233030,China;2.Department of Laboratory Medicine,the 82nd Hospital of PLA, Huaian,Jiangsu 223001,China;3.Institute of Pediatrics,Nanjing Medical University,Nanjing, Jiangsu 210029,China;4.Department of Child Care Health,Nanjing Maternal and Child Care Health Hospital Affiliated to Nanjing Medical University,Nanjing,Jiangsu 210004,China) |
| Abstract: | Objective To predict targets and functions of hsa-miR-100 by bioinformatics analysis,so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-100 biological function.Methods Current studies of miR-100 were retrieved by Pubmed.The sequence of hsa-miR-100 was got from miRBase database,NCBI,UCSC Browser.Target genes of hsa-miR-100 were predicted by miRanda,TargetScan and PicTar,and then analyzed by gene ontology and pathway enrichment analysis.Results Current studies showed that hsa-miR-100 was related with multiple tumorigenesis.miR-100 was highly conserved among different species.Gene ontology analysis demonstrated the target genes of hsa-miR-100 were enriched in gene silencing,chromatin silencing,negative regulation of biosynthetic process and other biological processes(P<0.01).The pathway analysis indicated the target genes mainly involved in cancer signaling,lysosome signaling,apoptosis signaling and other pathways(P<0.05).Conclusion The target genes of hsa-miR-100 enriched in multiple biological processes were relevant to the tumor signaling pathways. |
| Keywords: | microRNAs hsa-mir-100 bioinformatics analysis target genes neoplasm |
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