Measurement of lysophospholipase D/autotaxin activity in human serum samples |
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| Authors: | Nakamura Kazuhiro Ohkawa Ryunosuke Okubo Shigeo Tozuka Minoru Okada Mitsumasa Aoki Shinya Aoki Junken Arai Hiroyuki Ikeda Hitoshi Yatomi Yutaka |
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| Affiliation: | Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan. |
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| Abstract: | OBJECTIVES: Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. DESIGN AND METHODS: LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. RESULTS AND CONCLUSIONS: It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects. |
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