| HLA新基因B"非汉字符号"610携带者家系B淋巴细胞系的建立 |
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| 引用本文： | 李桢,邹红岩,吴国光,程良红,王大明,唐斯.HLA新基因B"非汉字符号"610携带者家系B淋巴细胞系的建立[J].中国输血杂志,2004(5). |
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| 作者姓名： | 李桢 邹红岩 吴国光 程良红 王大明 唐斯 |
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| 作者单位： | 深圳市血液中心输血医学研究所 广东深圳518035
(李桢，邹红岩，吴国光，程良红，王大明)，深圳市血液中心输血医学研究所 广东深圳518035(唐斯) |
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| 摘 要： | 目的 建立HLA新基因B 5 6 10携带者家系的B淋巴细胞系 ,用于该基因的研究和确证。方法 采集外周血分离淋巴细胞 ,用EBV感染淋巴细胞后 ,加入含 2 0 %FBS、2 μg/mlCsA的RPMI16 4 0进行培养。 结果 5位家庭成员的无限增殖化B淋巴细胞系新基因稳定遗传。结论 建立的无限增殖化B淋巴细胞系的HLA新基因B 5 6 10遗传稳定 ,为大量保存和繁殖珍贵的生物医学材料奠定了基础。
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| 关 键 词： | 人类白细胞抗原 EB病毒 胎牛血清 环孢菌素A B淋巴细胞系 |
Prepae B-lymphoblastoid cell lines of HLA novel allele B ~*5610 in a family |
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| LI Zhen,ZOU Hongyan,WU Guoguang,et al..Prepae B-lymphoblastoid cell lines of HLA novel allele B ~*5610 in a family[J].Chinese Journal of Blood Transfusion,2004(5). |
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| Authors: | LI Zhen ZOU Hongyan WU Guoguang |
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| Institution: | LI Zhen,ZOU Hongyan,WU Guoguang,et al.Shenzhen Institute of Transfusion Medicine,Shenzhen 518035,China |
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| Abstract: | Objective To prepare B-lymphoblastoid cell lines of HLA novel allele B*5610 in a family for further study and identification . Method Isolate mononuclear cells under aseptic conditions from the peripheral blood. After infection with Epstein-Barr virus, the cells were cultured in 20% FBS, 2μg/ml CsA RPMI 1640. Results Immortalized B-lymphoblastoid cell lines of five B *5610 carriers in a family were achieved, and the new genes were inherited stably. Conclusion Our work is important for storing and breeding the precious material of biomedicine because the B *5610 genes in the immortalized B-lymphoblastoid cell lines were inherited stably. |
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| Keywords: | HLA (Human leukocyte antigen EBV (Epstein-Barr virus) FBS (fetal bovine serum) CsA (CyclosporinA) |
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