锁阳化学成分的分离与鉴定

目的研究中药锁阳的化学成分。方法采用开放硅胶柱色谱、Sephadex LH-20、ODS等方法分离化合物,运用现代波谱技术确定化合物的结构。结果从锁阳乙醇提取物的乙酸乙酯萃取部分分离纯化得到5个化合物,分别鉴定为4-甲基邻苯二酚(4-methylcatechol,1)、3,4-二羟基苯甲酸乙酯(3,4-dihydroxy-benzoic acid ethyl ester,2),epiphyllocoumarin(3),(-)-落叶脂素[(-)-lariciresinol,4],dihydroapigenin(5)。结论化合物1-4为首次从锁阳科植物中得到,化合物5为首次从锁阳植物中得到。

Isolation and identification of chemical constituents from Cynomorium songaricum

Objective To study chemical constituents from Cynomorium songaricum．Methods The compounds were isolated by chromatography on silica gel column、, Sephadex LH-20,、 ODS and identified by physicalchemical physicochemical properties and spectrascopicspectroscopicl analysis．Results Five compounds were obtained and identified as 4-methylcatechol (1), 3,4-dihydroxy-benzoic acid ethyl ester(2), epiphyllocoumarin(3), ( -)-Lariciresinol (4), dihydroapigenin (5)． Conclusions Compounds 1, 2, 3 and 4 were are isolated from Cynomoriaceae for the first time, and compound 5 was is isolated from Cynomorium songaricum for the first time.