纳米化疗药物对胆管癌细胞中Survivin和p53表达的调节

目的 通过检测纳米化疗药物干预人胆管癌细胞株QBC939中Survivin和p53表达变化的情况,探讨其抑制肿瘤的机制.方法 体外培养人胆管癌细胞株QBC939,随机分成5组,即空白对照组、纳米药囊组、有磁无药组、5-FU组和吉西他滨(健择)组.采用MTT法和流式细胞术检测各组在药物干预后的细胞生长状态,并通过反转录一聚合酶链反应(RT-PCR)和Weslern blot检测各组中Survivin和p53 mRNA和蛋白的表达情况.结果 空白对照组、有磁无药组、5-FU组、健择组、纳米药囊组中Survivin mRNA和蛋白表达依次降低,p53则相反;纳米药囊组对QBC939细胞生长抑制明显,细胞凋亡率较其他各组高(除健择组).上述结果差异均有统计学意义(P＜0.05).结论 纳米化疗药物对人胆管癌细胞株QBC939有明显抑制作用,下调Survivin和上调p53可能是其抑瘤机制之一.

Expression of Survlvin and p53 in human biiiary tract carcinoma cell line regulated by nanochemotherapy drug

Objective To determine the effect of nanochemotherapy drug on Survivin and p53 ex-pressed by human biliary traet carcinoma cell line QBC939.Methods Culturing the human biliary tract carcinoma cell line QBC939 in vitro and it was divided into five groups including saline,nanochemotherapy drug,nanopartiele withoul nanochemotherapy drug,5-FU and gemcitabine.Using the methods of MTT and flow cytometry to observe the growth of QBC939 which was dealt with different drugs.In addition,RT-PCR and Western blot were used to delect the expression of mRNA and protein by Survivin or p53.Results The expression of mRNA and protein by Survivin decreased in the following set:saline,nanoparlicle withoul nanochemotherapy drug,5-FU,gemcitabine and nanochemotherapy drug,respeclively.However,the ex-pression of mRNA and protein by p53 were in reverse order.The inhibiting action to QBC939 was obvious in nanochenmtherapy drng and the apoplotic rate was higher than others except for gemcitabine(P＜0.05). Conclusion Nanochemotherapy drug has significant effect on treatment cholangiocarcinoma in vilro,which may attribute to the down regulation of Survivin and up regulation of p53.