高效液相色谱法测定三七血伤宁散中三七皂苷R1、人参皂苷Rg1和Rb1的含量

目的建立高效液相色谱法测定三七血伤宁散中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量。方法采用色谱柱ODSHypersil C18柱（4．6mm×250mm，5μm），以乙腈（A）-水（B）为流动相，进行梯度洗脱（0～25min，20％A，25-75min，20％～35％A），流速：1．0mL·min^-1，柱温：35℃，检测波长：203nm。结果三七皂苷R1在0．225-5．625μg与峰面积线性关系良好，r=1．0000，平均加样回收率为100．06％，人参皂苷Rg1在2．076=20．76μg与峰面积线性关系良好，r=0．9997，平均加样回收率为99．92％，人参皂苷Rb1在2．056～20．56魑与峰面积线性关系良好，r=0．9998，平均加样回收率为96．91％。结论本法准确可靠、灵敏度高、重现性好，可作为该产品质量控制的方法。

Determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Sanqixueshangning powder by HPLC

Objective To establish an HPLC method for the determination of notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rb1 in Sanqixueshangning powder. Methods The analytical column was an ODS Hypersil ClS （4. 6 mm × 250 mm, 5 μm）. The mobile phase consisted of acetonitrile （A） water （B） with the gradient elution （0-25 min, 20%A, 25-75 min, 20%-35%A）. The flow rate was 1.0 mL · min^-1 and the column temperature was 35 ℃. The detection wavelength was 203 nm. Results The linear range of notoginsenoside R1 was 0. 225 - 5. 625 μg with r= 1. 000 0, and the average sample recovery was 100.06%. The linear range of ginsenoside Rg1 was at 2. 076-20. 76μg with r=0. 999 7, and the average sample recovery was 99. 92%. The linear range of ginsenoside Rb1 was at 2. 056- 20. 56μg with r=0. 999 8, and the average sample recovery was 96.91%. Conclusion This method is accurate, sensitive and reproducible. It can be used for the quality control of Sanqixueshangning powder.