食管鳞癌组织端粒酶亚基的表达及端粒酶活性的关系

目的　探讨食管鳞癌组织中端粒酶活性、端粒酶逆转录酶 (h TERT)及端粒酶相关蛋白 - 1(TP- 1)的表达及其关系。方法　应用 TRAP-银染法对 45例食管鳞癌组织端粒酶活性的检测 ,同时应用原位杂交对癌组织切片进行 h TERT、TP- 1的 m RNA表达的检测。结果　癌组织端粒酶活性阳性率为 82 .2 %。癌组织中不同分化程度的端粒酶活性差异无显著性 (P>0 .0 5 )。有淋巴结转移者癌组织端粒酶活性明显高于无淋巴结转移者 ,差异有显著性(P<0 .0 5 )。癌组织中 h TERTm RNA表达的阳性率为 6 4.4%,TP- 1的阳性率为 6 2 .2 %。 h TERT的 m RNA表达与端粒酶活性密切相关 ,而 TP- 1的 m RNA表达与端粒酶活性无相关。结论　食管鳞癌组织中端粒酶活性及h TERT、TP- 1的 m RNA表达均较高。端粒酶活性与淋巴结转移有关。 h TERT与端粒酶活性有密切关系。

Correlation between expression of human telomerase subunits and telomerase activity in esophageal squamous cell carcinoma

Objective To investigate the telomerase activity and hTERT, TP-1 expression and their relationships in esophageal squamous cell carcinoma(ESCC). Methods The telomerase activity was measured in 45 specimens of ESCC tissues using TRAP assay by silver staining. In situ hybridization was used for detecting hTERT and TP-1 mRNA. Results The telomerase activity was detected in 82.2% of ESCC tissues. The difference of telomerase activity was not significant between high and low differential lesion (P>0.05). The positive rate of telomerase activity was higher in cases with lymphatic metastasis than in those without lymphatic metastasis (P<0.05). In cancer tissues the hTERT mRNA expression was 64.4% and the TP-1 mRNA expression was 62.2%. The expression of hTERT mRNA had correlation with telomerase activity, but TP-1 had not correlation with it. Conclusion The telomerase activity and hTERT, TP-1 mRNA expression are up-requlated in ESCC. The telomerase activity in ESCC tissues may be related to lymphatic metastasis and hTERT is correlated with telomerase activity.