Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing Escherichia coli Strains from Calves |
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| Authors: | Sophia M. Franck Brad T. Bosworth Harley W. Moon |
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| Affiliation: | Department of Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011,1. and Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 500102. |
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| Abstract: | A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves. |
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