| 山东荣成八代按蚊分类地位的探讨 |
| |
| 引用本文： | 周晓俊,施文琦,张仪,岳爱萍. 山东荣成八代按蚊分类地位的探讨[J]. 中国寄生虫学与寄生虫病杂志, 2011, 0(6) |
| |
| 作者姓名： | 周晓俊 施文琦 张仪 岳爱萍 |
| |
| 作者单位： | 中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室世界卫生组织疟疾血吸虫病和丝虫病合作中心;山东省荣成市疾病预防控制中心; |
| |
| 基金项目： | “十一五”国家科技支撑计划重大项目(No.2008ZX10004-010)~~ |
| |
| 摘 要： | 目的探讨山东荣成八代按蚊的分类地位。方法于2009年8月底在山东荣成西郊王家村采集饱血八代按蚊,饲养得子代,对子代成蚊进行形态学鉴定。抽提各型成蚊足的DNA,PCR扩增rDNA-ITS2片段后测序,并用DNAstar7.1软件对所获荣成八代按蚊的rDNA-ITS2序列与文献记录的山东荣成八代按蚊(YSD,AY306128)、四川省八代按蚊(YSC,AY170925)、辽宁省八代按蚊(YLN,AY170923)和韩国八代按蚊(YK,AF146749)的相关序列进行多重比对。结果共采集饱血八代按蚊56只,其中7只顺利产卵,饲养共获得子代成蚊354只,包括八代按蚊型(Y型,成蚊翅脉端白斑和V5.2顶繸白斑均有)240只、近黑按蚊型(P型,两者均无)8只和杂合型(H型,两者其一)106只。亲本和子代成蚊各型间rDNA-ITS2段序列(JN865249,JN865246,JN865247,JN865248)同源性为98.7~100%,与四川省八代按蚊、辽宁省八代按蚊和韩国八代按蚊的rDNA-ITS2段序列同源性分别为98.5%~99.3%、98.7%~99.6%和98.7%~100%,而与原山东荣成八代按蚊同源性仅为6...
|
| 关 键 词： | 荣成 八代按蚊 近黑按蚊 分类地位 |
Taxonomic Status of Anopheles yatsushiroensis in Rongcheng City of Shandong Province |
| |
| ZHOU Xiao-jun ,SHI Wei-qi ,ZHANG Yi ,YUE Ai-ping. Taxonomic Status of Anopheles yatsushiroensis in Rongcheng City of Shandong Province[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2011, 0(6) |
| |
| Authors: | ZHOU Xiao-jun SHI Wei-qi ZHANG Yi YUE Ai-ping |
| |
| Affiliation: | ZHOU Xiao-jun 1,SHI Wei-qi 1,ZHANG Yi 1,YUE Ai-ping 2 (1 National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Key Laboratory of Parasite and Vector Biology,MOH,WHO Collaborating Centre for Malaria,Schistosomiasis and Filariasis,Shanghai 200025,China,2 Center for Disease Control and Prevention of Rongcheng City,Rongcheng 264300,China) |
| |
| Abstract: | Objective To clarify the taxonomic status of Anopheles yatsushiroensis in Rongcheng City of Shandong Province.Methods By the end of August 2009,Anopheles yatsushiroensis were collected in the western suburbs of Wangjia Village of Rongcheng City,and raised for the next generation of various types of adult mosquitoes.Through morphological identification of filial generation various types,mosquito feet of 1-2 mosquitoes of various types were taken for complete DNA extraction,and complete sequence analysis of r... |
| |
| Keywords: | Rongcheng Anopheles yatsushiroensis Anopheles pullus Taxonomy |
| 本文献已被 CNKI 等数据库收录！ |
|
