Antenatal Saireito (TJ‐114) Can Improve Pulmonary Hypoplasia and Pulmonary Vascular Remodeling in Nitrofen‐Induced Congenital Diaphragmatic Hernia |
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Authors: | Shima Hirako Hiroyuki Tsuda Tomomi Kotani Seiji Sumigama Yukio Mano Tomoko Nakano Kenji Imai Hua Li Shinya Toyokuni Fumitaka Kikkawa |
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Institution: | 1. Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya, Japan;2. Bell Research Center for Reproductive Health and Center, Nagoya, Japan;3. Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, Japan |
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Abstract: | Congenital diaphragmatic hernia (CDH) can induce lung hypoplasia and pulmonary hypertension and is associated with high mortality. The purpose of this study is to examine the efficacy and safety of antenatal Saireito (TJ‐114), a traditional Japanese herbal medicine, in a rat CDH model. Sprague‐Dawley rats were exposed to an herbicide (nitrofen, 100 mg) on embryonic day 9 (E9) to induce CDH, and antenatal Saireito (2000 mg/kg/day) was orally administered from E10 to E20. On E21, fetuses were delivered. Antenatal Saireito significantly decreased the incidence of CDH (p < 0.01), increased lung volume (p < 0.01), improved alveolarization and pulmonary artery remodeling using histological analysis, and improved respiratory function using gasometric analysis (pH; p < 0.05, and PCO2; p < 0.01). In addition, antenatal Saireito significantly decreased endothelin‐1 and endothelin receptor A expression in the pulmonary arteries. Taken together, our results demonstrated that antenatal Saireito can improve fetal pulmonary hypoplasia and pulmonary vascular remodeling and, as a result, can improve respiratory function in a rat CDH model. Copyright © 2016 John Wiley & Sons, Ltd. |
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Keywords: | alveolarization congenital diaphragmatic hernia endothelin‐1 pulmonary artery remodeling respiratory function Saireito |
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