64层螺旋CTPA在肺栓塞动物实验研究中的诊断价值评估

目的探讨64层螺旋CT在肺动脉栓塞诊断中的价值,为进一步临床研究提供有效依据。方法成年健康中国大耳白兔10只,体重2.5～3.5kg,抽取耳缘静脉血1ml制备自体血栓后建立静脉通路,进行CT血管造影（CTPA）基础数据采集,将血栓经颈外静脉通路注入实验兔体内再次进行64层螺旋CT肺动脉造影,记录血栓位置及数目,扫描后将其处死,解剖并记录血栓位置及数目。结果 10只实验兔其中1只由于栓塞过量未取得数据,其余9只实验兔均成功地制成肺栓塞动物模型。其中64层螺旋CT对于血管直径〈2.0mm以上肺动脉共检出血栓33处,准确率达100%。1.5～2.0mm直径肺动脉检出血栓29处,病理解剖显示32处,检出敏感性为84.38%,特异性为50.0%。肺动脉直径〈1.5mmCTPA检出敏感性47.39%,特异性93.18%。结论 64层螺旋CT肺动脉造影对肺动脉栓塞的诊断,特别是对周围型肺栓塞的影像学诊断的优势确切,可以广泛应用于临床。

Diagnostic value of 64-slice spiral CT pulmonary angiography for pulmonary embolism in animal experiment

Objective To evaluate the value of the 64-slice spiral CT pulmonary angiography（64-SCTPA）in the diagnosis of pulmonary embolism（PE）by observing the reveal rate in rabbit models,and to provide the basic date for clinical study.Methods The 1ml blood was taken from the ear vein of 10 healthy Chinese white rabbits weighted 2.5～3.5kg,then transcathetered via the left jugular vein to collect the basic data of CTPA.The autologous blood clots were injected into the left jugular vein,then 64-SCTPA was performed to get the location and number of PE.The rabbits were killed for pathological examination,and the examination results were compared with the imaging of 64-SCTPA before and after the embolism.Results There was only 1 animal failed to collect the data because of excessive embolism,the models of PE were successfully established in the other 9 animals.There were 33 embolisms were found by 64-SCT,which located in the pulmonary artery whose diameter was over 2mm,and the sensibility and the specificity are 100%;32 embolisms located in the pulmonary artery whose diameter was between 1.5～2.0mm in which there were 29 embolisms detected by CT,the sensibility was 84.38% and the specificity was 50%;for the vascular diameter was less than 1.5mm,the sensibility was 47.39% and specificity was 93.18%.Conclusion 64-SCTPA has an advantage in the diagnosis of PE,especially for the periphery embolism of pulmonary artery,which can be used in clinic widely.