卵巢癌中肝细胞生长因子的表达与其微血管密度及淋巴管密度的关系

目的:探讨肝细胞生长因子(HGF)在上皮性卵巢癌组织中的表达及其与肿瘤血管生成及淋巴管生成的关系。方法:应用免疫组化S-P法检测HGF在上皮性卵巢癌、交界性卵巢上皮性肿瘤和良性卵巢上皮性肿瘤中的表达;用CD34标记卵巢癌组织中的微血管,单克隆抗体D2-40标记卵巢癌组织中淋巴管,采用图像分析进行微血管密度(MVD)和微淋巴管密度(LVD)测定。结果:HGF表达阳性率在卵巢癌中最高。HGF、MVD、LVD表达与卵巢癌FIGO分期、腹膜种植、远处转移及淋巴结转移显著相关(P<0.05),且HGF表达与MVD、LVD有显著相关性。结论:HGF可促进卵巢微血管及淋巴管生成,并参与肿瘤侵袭和转移过程,检测HGF的表达及MVD、LVD可作为卵巢癌侵袭、转移等生物行为及估计预后的有意义指标。

Relationship between expression of hepatoeyte growth factor and microvessel density, microlymphatic density in ovarian carcinoma

Objective:To explore the expression of hepatocyte growth factor(HGF) in epithelial ovarian carcinoma and the relationship between HGF and tumor angiogenesis,lymphangiogenesis.Methods:The expression levels of HGF in patients with epithelial ovarian carcinoma,borderline epithelial ovarian carcinoma and benign epithelial ovarian carcinoma were detected by immunohistochemistry,respectively. Microvessels in ovarian carcinoma were marked by CD34 and lymphatic microvessels in ovarian carcinoma were marked by monoclonal antibody D2-40,then microvessel density(MVD) and microlymphatic density(LVD) were detected by image analysis.Results: The positive rate of HGF in ovarian carcinoma was the highest.There was a correlation between HGF,MVD,LVD and FIGO staging,peritoneal seeding,distant and lymphatic metastasis(P<0.05),and there was a correlation between expression of HGF and MVD, LVD.Conclusion:HGF can promote lymphangiogenesis and microangiogenesis,and participate in the invasion and metastasis of ovarian carcinoma.The detection of HGF,MVD and LVD can be used as a indicator of biological behaviors and prognosis of ovarian carcinoma.