内皮抑素质粒联合顺铂对上皮性卵巢癌细胞株A2780增殖和凋亡的影响

目的:探讨内皮抑素质粒联合顺铂对上皮性卵巢癌细胞株A2780增殖和凋亡的影响。方法:通过瞬时转染内皮抑素质粒到卵巢癌细胞A2780后,采用MTT法和流式细胞术检测内皮抑素以及内皮抑素和顺铂联合应用对细胞增殖和凋亡的影响。结果:MTT法检测发现转染内皮抑素质粒后,A2780细胞的增殖明显减慢,顺铂对其生长抑制作用明显增强,差异有统计学意义(P<0.05)。流式细胞仪检测发现与空白组相比,转染内皮抑素质粒后A2780细胞的G0/G1期细胞明显增加,S期细胞明显减少,细胞的凋亡率由(1.59±0.86)%增加到(8.66±1.27)%;内皮抑素质粒联合顺铂治疗后,上述差异更加显著(P<0.01)。结论:内皮抑素联合顺铂可通过抑制A2780细胞的生长和增加其凋亡而产生协同抗瘤作用。

Effect of endostatin plasmid combined with cisplatin on the proliferation and apoptosis of human ovarian cancer cell line A2780

Objective:To explore the effect of endostatin plasmid combined with cisplatin on the proliferation and apoptosis of human ovarian cancer cell line A2780.Methods:After the transfection of endostatin plasmid into A2780 cells,MTT and flow cytometry were used to detect the effects of endostatin or endostatin+cisplatin on the proliferation and apoptosis of A2780 cells.Results:MTT assay revealed that the inhibition of cisplatin to the growth of A2780 cells transfected with endostatin plasmid was significantly enhanced,compared with control group,the proliferation reduced significantly (P<0.05).The flow cytometry revealed that after transfection of endostatin plasmid into A2780 cells,compared with control group,the apoptosis rate increased from (1.59±0.86)% to (8.66±1.27)%,and the G0/G1 stage cell rate increased significantly,the S stage cell rate reduced sharply and apoptosis rate increased significantly (P<0.05).In addition,after combined use of endostatin plasmid and cisplatin,all the changes were more significant (P<0.01).Conclusion:Endostatin can effectively inhibit the growth and promote the apoptosis of ovarian cancer cell lines A2780,combined endostatin with cisplatin have synergic effect on anti-ovarian cancer therapeutics.