RhoC基因对宫颈癌细胞粘附和侵袭能力的影响 |
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引用本文: | 贺晓琪,肖勇,钱颖,蔡惠兰,任利容,王泽华. RhoC基因对宫颈癌细胞粘附和侵袭能力的影响[J]. 中国妇幼保健, 2009, 24(26): 3726-3728 |
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作者姓名: | 贺晓琪 肖勇 钱颖 蔡惠兰 任利容 王泽华 |
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作者单位: | 1. 华中科技大学同济医学院附属协和医院妇产科,湖北,武汉,430022 2. 华中科技大学同济医学院附属协和医院普外科 |
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摘 要: | 目的:研究RhoC对宫颈癌细胞粘附、侵袭和迁移能力的影响。方法:以宫颈癌SiHa细胞株为研究对象,转入RhoC小干扰RNA抑制其表达,通过体外粘附实验、侵袭实验和迁移试验比较SiHa细胞粘附率、侵袭能力和迁移能力的变化。结果:转入RhoC小干扰RNA后,RhoC蛋白的表达明显下调,对细胞外基质的粘附率明显下降(P<0.01),体外侵袭能力和迁移能力明显下降,侵袭抑制率和迁移抑制率分别为(19.94±6.06)%和(19.16±5.26)%。结论:RhoC能明显促进宫颈癌的转移。
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关 键 词: | RhoC RNA干扰 粘附 侵袭 迁移 |
RhoC siRNA inhibits adhension and invasion of human cervical cancer Siha cell line in vitro |
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Abstract: | Objective:To investigate the roles of RhoC to adhension,invasion and migration of cervical cancer.Methods:To transfect RhoC siRNA to downregulate the expression of RhoC.To test the adhensive ratio,invasive capability an migratory capability by adhesive assay,invasive assay and migratory assay.Results:Expression of RhoC was downregulated significantly after the RhoC siRNA was transfected.The adhesive ratio was decrease(P<0.01).The invasive capability and migratory capability were depressed.The invasive inhibition ratio was(19.94±6.06)% and the migratory inhibition ratio was(19.16±5.26)%.Conclusion:RhoC facilitates metastasis of cervical cancer. |
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Keywords: | RhoC RNA interfere Adhension Invasion Migration |
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