野山楂根拮抗雷公藤多苷对雄性大鼠生殖损伤作用的研究

目的:观察中药野山楂根水煎剂对雷公藤多苷所致雄性不育大鼠生育力的影响。方法:将成年雄性大鼠随机分组,分别给予雷公藤多苷片溶液(10 mg.kg^-1),野山楂根水煎剂(1.8,5.4,18 g.kg^-1)+雷公藤多苷片溶液(10 mg.kg^-1)和蒸馏水(1 mL.kg^-1)灌胃,8周后停用雷公藤多苷,中药组继续行野山楂根灌胃。4周后各组动物与成年雌性大鼠交配,观察交配雌鼠妊娠率和平均胚胎、黄体数。测定雄鼠生殖器官性腺脏器指数、附睾精子计数、活力、存活率,观察各组动物睾丸、附睾组织病理和超微结构改变,放射免疫法测定血清和睾丸组织睾酮(T),血清卵泡刺激素(FSH)和促黄体生成素(LH)含量。结果:与雷公藤多苷模型组比较,野山楂根组交配雌鼠妊娠胎数、雄鼠睾丸、附睾脏器指数以及附睾精子计数、活力均有提高(P<0.05);野山楂根组精子超微结构较模型组有明显恢复;睾丸组织匀浆T显著高于模型组(P<0.01)。结论:野山楂根能够拮抗雷公藤多苷对雄性大鼠的生殖损伤作用,提高不育症模型雄鼠的生育力。

Resistant effect of water decoction of root of Crataegus cuneata on male infertility induced by GTW in rats

Objective: To observe the effects of water decoction of the root of Crataegus cuneata on infertility induced by multi-glucoside of Tripterygium wilfordii(GTW) in rats.Method: Male adult rats were randomly divided into five groups,which were treated via gastric gavage of distilled water(1 mL·kg^-1),solution of GTW(10 mg·kg^-1) and three doses of water decoction of root of C.cuneata(1.8,5.4,18 g·kg^-1) + GTW(10 mg·kg^-1),respectively.8 weeks later,GTW was stopped and the decoction and water continued for another 4 weeks.And then,all the male rats were copulated with adult female rats.The rates of pregnancy,average numbers of embryos and luteum of female rats,relative weights of reproductive organs,sperm counts,sperm motility and viability were compared among all the groups.The histology and ultrastructure of testis and epididymis were observed,while the concentrations of follicle stimulating hormone(FSH),luteinizing hormone(LH) and testostorone(T) in serum and T in testicular homogenate were detected by radioimmunoassay.Result: Compared with those in GTW model group,the embryo numbers,the relative weight of testis and epididymis and sperm counts and motility in C.cuneata groups were increased obviously(P<0.05).After treatment,the morphological damages of seminiferous tubules and sperms were recovered,while concentrations of T in testicular homogenate were also significantly increased(P<0.01).Conclusion: C.cuneata could relieve the reproductive lesions induced by GTW,and hence improve the uberty of the male infertile model rats.