| 应用PCR法检测副溶血性弧菌 |
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| 引用本文： | 詹铭,陈纯,沈小明,张勇琪,李婷,丁明. 应用PCR法检测副溶血性弧菌[J]. 实用预防医学, 2005, 12(3): 476-478 |
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| 作者姓名： | 詹铭 陈纯 沈小明 张勇琪 李婷 丁明 |
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| 作者单位： | 上海市浦东新区疾病预防控制中心,上海,200135 |
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| 基金项目： | 上海市疾病预防控制中心立项课题（区2001-12） |
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| 摘 要： | 目的 研究副溶血性弧菌的PCR检测方法。方法 引物选择副溶血性弧菌耐热溶血素（TDH）上的基因，应用PCR法，检测副溶血性弧菌。结果 PCR能检测经选择性增菌液培养的副溶血性弧菌，扩增片段在430bp：结论 应用PCR检测副溶血性弧菌，具有快速、特异、灵敏和简便的特点。
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| 关 键 词： | 副溶血性弧菌 PCR 检测 |
| 文章编号： | 1006-3110（2005）03-0476-03 |
| 收稿时间： | 2004-12-05 |
| 修稿时间： | 2004-12-05 |
Detection of Vibrio Parahemolyticus by Polymerase Chain Reaction |
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| ZHAN Ming , CHEN Chun , SHEN Xiao - Ming ,et al.. Detection of Vibrio Parahemolyticus by Polymerase Chain Reaction[J]. Practical Preventive Medicine, 2005, 12(3): 476-478 |
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| Authors: | ZHAN Ming CHEN Chun SHEN Xiao - Ming et al. |
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| Affiliation: | Shanghai Pudong New Area Center for Disease Control and Prevention, Shanghai 200135 |
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| Abstract: | Objective To study the method for detection of Vibrio parahemolyticus by polymerase chain reaction (PCR). Method Based on unique sequence characteristic of thermostable direct hemolysin (TDH), TDH was amplified by polymerase chain reaction. Results TDH primers targeted a 430bp region of TDH gene specific to Vibrio parahemolyticus. Conclusion PCR based on unique sequence characteristic of TDH is a method of high specificity, good (reproducibility) and high sensitivity. It's a practical method for Vibrio parahemolyticus detection. |
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| Keywords: | Vibrio parahemolyticus POR Detection |
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