|
|||||
|
|
| 雷公藤甲素对血管生成的抑制作用 | |
| 引用本文： | 丁怡,张建成,侯立军,张杰,王秋林,王树人.雷公藤甲素对血管生成的抑制作用[J].生物医学工程学杂志,2005,22(4):778-781. |
| 作者姓名： | 丁怡 张建成 侯立军 张杰 王秋林 王树人 |
| 作者单位： | 1. 四川大学,华西基础与法医学院,病理生理教研室,成都,610041 2. 四川大学,华西医院,消化外科,成都,610041 3. 潍坊医学院,体育教研室,潍坊,261042 4. 潍坊医学院,眼科,潍坊,261042 |
| 基金项目： | 山东省教育厅资助课题(J01K08) |
| 摘 要： | 利用体外培养人脐静脉内皮细胞,经不同浓度的雷公藤甲素(0、5、10、20、30μg/L)处理后,MTT法显示雷公藤甲素可抑制内皮细胞的增殖,5μg/L雷公藤甲素的抑制率达29.15%;琼脂凝胶立体细胞培养系统检测发现内皮细胞经雷公藤甲素作用后,其游走能力降低;鸡胚尿囊膜试验观察到雷公藤甲素可有效抑制血管的生成;荧光定量RT-PCR检测发现雷公藤甲素可下调内皮细胞u-PAmRNA的表达。因此认为,雷公藤甲素可能在基因水平上干扰内皮细胞u-PAmRNA的表达,减少u-PA蛋白的生成,从而有效地抑制血管内皮细胞的增殖和移行,这可能是雷公藤甲素抑制血管生成的主要机制之一。 |
| 关 键 词： | 雷公藤甲素 内皮细胞 尿激酶型纤溶酶原激活物 血管生成 荧光定量RT-PCR |
| 收稿时间： | 2005-01-06 |
| 修稿时间： | 2005-01-062005-03-18 |
Effects of Triptolide on Anti-angiogenesis by Reducing Expression of Urokinase Plasminogen Activator |
|
| Ding Yi,ZHANG Jiancheng,HOU Lijun,Zhang Jie,Wang Qiulin,WANG Shuren.Effects of Triptolide on Anti-angiogenesis by Reducing Expression of Urokinase Plasminogen Activator[J].Journal of Biomedical Engineering,2005,22(4):778-781. | |
| Authors: | Ding Yi ZHANG Jiancheng HOU Lijun Zhang Jie Wang Qiulin WANG Shuren |
| Institution: | Department of Pathophysiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China. |
| Abstract: | In order to unveil the anti-angiogenic mechanism of triptolide, we investigated the effects of triptolide on the proliferation, migration, angiogenesis and u-PA expression of cultured HUVECs. MTT assay found that triptolide could inhibit the proliferation of HUVECs,and three-dimensional culture system revealed that triptolide could curb the migration of HUVECs. The chick embryo chorioallantoic membrane (CAM) test showed that triptolide could inhibit angiogenesis. Real time quantitive RT-PCR showed the expression of u-PA of HUVECs was down-regulated by triptolide. Therefore, triptolide may inhibit the proliferation and migration of endothelial cell by way of reducing the expression of u-PA. These findings may conduce to the elucidation of the anti-angiogenic mechanism of triptolide. |
| Keywords: | Triptolide Endothelium u-PA Angiogenesis Real-time quantitative RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |