Effect of membrane protein concentration on binding of 3H-imipramine in human platelets |
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| Authors: | A I Barkai S Kowalik M Baron |
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| Affiliation: | 1. Bioceramics Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala 695 012, India;2. Department of Chemistry, National Institute of Technology, Calicut, Kerala 673601, India;1. Warren Alpert Medical School, Brown University, Providence, RI, United States of America;2. Harvard Department of Psychology, Harvard University, Cambridge, MA, United States of America;3. Center for Prisoner Health and Human Rights, Providence, RI, United States of America;4. Brown University School of Public Health, Brown University, Providence, RI, United States of America;5. Rhode Island Department of Corrections, Cranston, RI, United States of America |
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| Abstract: | Binding of 3H-imipramine to platelet membranes has been implicated as a marker for depression. Comparing 3H-IMI binding between depressed patients and normal subjects we observed an increase in the dissociation constant Kd with increasing membrane protein. This phenomenon was studied more rigorously in five normal subjects. Platelet membranes were prepared and adjusted to four concentrations of protein ranging from 100 to 800 micrograms/ml. The 3H-IMI binding parameters of maximum binding sites number (Bmax) and Kd were obtained by Scatchard analysis at each membrane concentration. A positive linear relationship was found between Kd values and the concentration of membrane protein in the assay, but no change was observed in Bmax. The variability in Kd values reported in the literature may be accounted for in part by the different concentrations of membrane protein used in various studies. |
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