肝癌细胞核DNA含量的定量测定及其临床病理学意义

本文报告应用图像分析技术,对38例肝癌的细胞核DNA含量进行了定量测定。结果显示;肝癌DNA含量与肝癌瘤体大小、包膜突破、癌栓形成以及临床预后有明显相关性,但与临床分期及病理分级无关;66.7％的DNA二倍体/近二倍体(D/ND)肝癌瘤体直径≤3cm,而75％的DNA异倍体(AN)肝癌瘤体直径>3cm,提示肝癌直径在3cm时可能是其生物学特性转变的重要时期。本文认为,对肝癌DNA含量的定量分析,可为从形态学上估价肝癌的生物学特性提供有价值的补充,并为判断临床预后提供客观依据。

SIGNIFICANCE OF CLINICOPATHOLOGY IN QUANTITATIVE MEASUREMENT OF DNA CONTENT IN HEPATOCELLULAR CARCINOMA

DNA content of 38 hepatocellular carcinomas (HCC) was quantitatively determined by means of image analysis technology .The results showed that DNA content in HCC was correlated well with the tumor size, capsule breaking, cancerous thrombus forming and clinical prognosis, that is, the higher DNA content was, the more malignance appeared, but not with the clinical staging and pathological grading of HCC. 66.7% of DNA diploid/near diploid HCC were less than or equal to 3cm in diameter, whereas 75% of DNA aneuploid HCC were more than 3cm in diameter (P<0.05), suggesting that HCC at 3cm in size may be an important period when the change of biological characteristics would occur. This study indicates that the quantitative analysis of DNA content can provide a valuable supplement to morphology in predicting the biological features and objective basis for assessing clinical prognosis of HCC.