抗肿瘤抗生素1588的研究——Ⅰ.产生菌的生物学特征及抗生素1588的发酵、分离及纯化

我们用诱导前噬菌体的方法在上海的花园土壤中筛选到一株丁香轮丝链霉菌1588。本文报道该菌的生物学特征及其产生的抗生素1588的发酵、分离及纯化的研究结果。文中还介绍了一种适于对酸碱不稳定的腐草霉素—博莱霉素类抗生素的提取流程,也介绍了用一株对同时存在于抗生素1588发酵液中的其它抗生素不敏感的枯草杆菌进行生物测定的方法。

STUDIES ON ANTITUMOR ANTIBIOTIC 1588 Ⅰ. BIOLOGICAL CHARACTERISTICS OF PRODUCING STRAIN AND FERMENTATION ISOLATION AND PURIFICATION OF ANTIBIOTIC 1588

In screening of antitumor antibiotics a strain of streptomyces was isolated from garden soil in Shanghai, which produces an antibiotic belonging to the phleomycinbleomycin family. This strain has been identified as Streptomyces lilacinoverticillatus 1588 Yen et Zhang. The antibiotic produced by this strain was named Antibiotic 1588.In this paper, the biological characteristics of this strain and the selection of the fermentation media by the method of multiple-factor orthogonal experimental design were reported.An isolation-purification procedure for Antibiotic 1588 using strong acidic cation exchange resin, porous resin and active aluminum oxide chromatography was established.In the bioassay, Bacillus subtilis A. S. 1.311-8 was used as the testing bacteria. This strain was insensitive to other antibiotics exsisting in the culture broth besides Antibiotic 1588.