大鼠坐骨神经损伤后神经再生的形态学观察

目的 研究周围神经损伤后的再生情况。方法 切断并原位吻合右侧SD大鼠坐骨神经。左侧不作任何处理、作为对照。手术后不同时间取跨越吻合口两侧的神经段作纵向冰冻切片，行乙酰胆碱酯酶(AChE)组织化学染色，观察神经纤维的变化。同时取左侧一段神经作对照染色。结果 右侧坐骨神经损伤后。术后第1天至第7天无神经纤维通过吻合口。且神经纤维排列杂乱；第14天只有小部分神经纤维通过吻合口；第21天有部分神经纤维通过吻口；第30天有较多神经纤维通过吻合口；第45天有大量神经纤维通过吻合口。但排列不整齐；第60天吻合口神经纤维排列整齐。而左侧的神经纤维排列整齐、均匀。结论 大鼠坐骨神经切断损伤后，神经纤维先发生溃变，1个月后有大量神经能够再生，至2个月，再生神经纤维的排列近似正常。

Morphological observation of nerve regeneration following the rat sciatic nerve injury

Objective To study peripheral nerve regeneration after its injury in adult rats.Methods First of all,the right sciatic nerve of SD rat was cut and anastomosed with the left side remained orignal as a control.Furthermore,at different time after operation,the nerve segments which contain two parts of the cut were removed out and stained with AChE (acetylcholinesterase).Results After right sciatic nerve operation,from lst to 7th day,no nerve fiber passed the cut;on 14th day,only a little passed the cut;on 21st day,parts of nerve fibers passed the cut; on 30th day,more nerve fibers passed the cut;on 45th day,plenty of nerve fibers passed the cut,but irregularly;on 60th day,the nerve fibers passed the cut regularly.Conclusion The injured sciatic nerve degenerates at first,then it regenerates;on 2nd month after operation,plenty of nerve fibers regenerate and arrange regularly.