皮下注射血管内皮生长因子基因提高大鼠背部随意皮瓣活力的实验研究

目的 探索基因治疗在组织移植方面的方法。应用 构建并体外扩增表达质粒pcDNA3.1/Zeo( )-VEGF165，以之直接皮下注射转染SD大鼠背部随意皮瓣模型。通过与对照组相比较，观察其对皮瓣活力的影响，并采集组织行免疫组化检查。结果 基因转染组在毛细血管周围及肌间隙可见VEGF的沉积，与对照组相比皮瓣的平均成活面积显著增加。结论 皮下注射的基因在组织中能够表达VEGF，增加皮瓣活力，是一种简单有效的基因治疗方法。

Subcutaneous injection of plasmid VEGF gene: a method of gene therapy to enhance the viability of random skin flap

OBJECTIVE: Gene therapy has been becoming one of the most attractive medical areas. But the using of gene therapy in plastic surgery is relatively scarce. Our purpose was to investigate the effect of naked plasmid encoding Vascular Endothelial Growth Factor on the viability of the random skin flap by directly injected subcutaneously. METHODS: 30 female Sprague-Dawley rats randomly divided into three groups. A random dorsal skin flap of 3 cm x 9 cm was elevated in each of the rats. And 1 ml double-distilled water solution was injected subcutaneously, which was only water in group 1 during the operation, 200 micrograms VEGF cDNA plasmid in group 2 during the operation, 200 micrograms pcDNA3.1/zeo(+)--VEGF in group 3, 24 hours before the operation, respectively. 7 days after the operation, all the animals were sacrificed by overdose anesthetic. The survival tissue was measured with planimetry. Two samples were harvested from each group for pathological check and immunohistochemical test. RESULTS: Immunohistochemical staining demonstrated that there was human VEGF deposited around the capillary in the flaps treated with VEGF gene. The flaps treated with VEGF gene had a larger percentage of survival skin (group 1 = 47% +/- 5.4%, group 2 = 65.4% +/- 6.3%, group 3 = 72.3% +/- 8.5%, P < 0.05). CONCLUSION: VEGF gene directly injected into subcutaneous can express VEGF. It makes the gene therapy simple and practical and will be promising future in the tissue transplantation.