| 顺铂对A549细胞DNA修复酶MGMT和DNA-PKcs表达的影响 |
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| 引用本文： | 张霞 吕斌 郝巧玲 周宜开. 顺铂对A549细胞DNA修复酶MGMT和DNA-PKcs表达的影响[J]. 环境与职业医学, 2004, 21(1): 22-25 |
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| 作者姓名： | 张霞 吕斌 郝巧玲 周宜开 |
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| 作者单位： | 华中科技大学同济医学院环境医学研究所 湖北武汉430030(张霞，吕斌，郝巧玲)，华中科技大学同济医学院环境医学研究所 湖北武汉430030(周宜开) |
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| 基金项目： | 国家自然科学基金重大项目 (编号 :39990570) |
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| 摘 要： | [目的]探讨在DNA损伤剂顺铂作用下，A549细胞内DNA修复基因六氧甲基鸟嘌呤DNA甲基转移酶(MGMT)和DNA依赖的蛋白激酶复合物催化亚单位(DNA-PKcs)的表达变化。[方法]MTT法分析顺铂对A549细胞生存力的影响；彗星实验研究顺铂对DNA损伤的程度；RT-PCR分析不同顺铂作用时问修复酶mRNA水平的变化。[结果]顺铂对A549细胞的生长有明显的抑制作用，且为剂量依赖性。低浓度(IC20剂量)顺铂作用下，DNA损伤程度的变化与作用时间成正比，且伴随MGMT和DNA-PKcs的表达上升。停止作用后，DNA损伤程度减轻，修复基因的表达也逐渐恢复至正常水平。[结论]短期顺铂染毒所导致的急性DNA损伤对MGMT和DNA-PKcs的表达有明显的正性诱导作用。
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| 关 键 词： | 顺铂 A549细胞 DNA修复酶 MGMT DNA-PKcs DNA损伤 RT-PCR法 DNA修复系统 肿瘤细胞 |
| 文章编号： | 1006-3617(2004)01-0022-04 |
| 修稿时间： | 2003-01-03 |
Effects of Cisplatin on Expression of DNA Repair Genes:MGMT and DNA-PKcs in A549 Cells |
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| ZHANG Xia,LU Bin,HAO Qiao-ling,ZHOU Yi-kai. Effects of Cisplatin on Expression of DNA Repair Genes:MGMT and DNA-PKcs in A549 Cells[J]. Journal of Environmental & Occupational Medicine, 2004, 21(1): 22-25 |
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| Authors: | ZHANG Xia LU Bin HAO Qiao-ling ZHOU Yi-kai |
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| Abstract: | ObjectiveTo study the expression levels of MGMTand DNA-PKcs in A549cells treated with cisplatin.[Methods] The effect of cisplatin on the survival ability of A549cells,DNA damage level and the expression of DNA repair genes were examined using MTT,comet assay and RT-PCR methods respectively.[Results] Cisplatin induced a dose-dependent decrease in cell survival.When treated with cisplatin at IC 20 ,DNA damage level increased as cisplatin-treated time increased and the expression level of MGMT and DNA-PKcs increased too.DNA damage and the MGMT and DNA-PKcs level were turned gradually to normal level after cisplatin treatment was stopped.[Conclusion]Acute DNA damage induced by cisplatin in short time resulted in positive regulation of MGMT and DNA-PKcs expression in A549cells. |
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| Keywords: | MGMT DNA-PKcs Cisplatin A549cell line |
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