Cryopreservation of human mononuclear cells for quality control in clinical immunology. I. Correlations in recovery of K- and NK-cell functions,surface markers,and morphology |
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| Authors: | Douglas M. Strong John R. Ortaldo Franco Pandolfi Annette Maluish Ronald B. Herberman |
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| Affiliation: | (1) Naval Medical Research Institute, National Naval Medical Center, 20814 Bethesda, Maryland;(2) Uniformed Services University of the Health Sciences, 20814 Bethesda, Maryland;(3) Laboratory of Immunodiagnosis, National Cancer Institute, National Institutes of Health, 20205 Bethesda, Maryland;(4) Department of Clinical Immunology, University of Rome, Rome, Italy;(5) Transplantation Research Branch, Casualty Care Research Program Center, Naval Medical Research Institute, 20814 Bethesda, Maryland |
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| Abstract: | Cryopreserved human peripheral blood mononuclear cells (PBMC) were tested for natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC) and for high-affinity (29°C) and total (4°C) rosette formation with sheep erythrocytes. PBMC produced variable NK activity following freezing and thawing, but consistently reacted well in ADCC. A significant correlation was found between low NK activity and a decreased percentage of low-affinity rosette-forming cells. On the contrary, the number of large granular lymphocytes (LGL), among which NK cells are restricted, and the reactivity with the monoclonal antibody OKT10, which recognizes the majority of LGL in the peripheral blood, were not significantly altered by cryopreservation. Cryopreserved cells proved to be excellent controls for determining the day-to-day variability of the NK assay and for selecting optimum conditions for this test in the clinical immunology laboratory. |
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| Keywords: | Natural killer cells (NK) antibody-dependent cellular cytotoxicity (ADCC) cryopreservation cytotoxicity low-affinity rosettes high-affinity rosettes |
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