多囊卵巢综合征患者颗粒细胞AQP9的表达

目的:研究多囊卵巢综合征(polycystic ovarian syndrome,PCOS)卵巢组织中窦状卵泡和在超促排卵(COH)周期黄素化颗粒细胞AQP9的表达,探讨颗粒细胞AQP9表达水平与COH周期卵泡液中甾体激素水平的关系。方法:行体外受精-胚胎移植的PCOS患者18例,对照组18例。收集卵泡(直径≥1.8 cm)液,测定甾体激素E2、P和T水平;取卵时分别收集大卵泡(直径>1.6 cm)和小卵泡(直径<1.4 cm)的黄素化颗粒细胞。用RT-PCR检测COH周期黄素化颗粒细胞AQP9mRNA的表达,免疫组化定位AQP9在PCOS卵巢内窦状卵泡和COH周期的黄素化颗粒细胞的表达。结果:RT-PCR检测证实黄素化颗粒细胞有AQP9 mRNA表达,PCOS组AQP9 mRNA表达水平与对照组比较差异无显著性(P>0.05),PCOS组大卵泡的颗粒细胞AQP9表达高于小卵泡,但无统计学差异。免疫组化显示PCOS患者卵巢的窦状卵泡内颗粒细胞和COH周期的黄素化颗粒细胞均有AQP9的表达,染色定位于胞浆和胞膜。在COH周期,颗粒细胞AQP9 mRNA表达水平与卵泡液中E2、P和T均无显著性相关。结论:PCOS患者卵巢组织中窦状卵泡的颗粒细胞及在COH周期黄素化颗粒细胞均有AQP9表达,推测AQP9可能通过水转运介导卵泡发育和窦卵泡形成。在COH周期,PCOS的大卵泡AQP9的表达有高于小卵泡的趋势,可能与卵泡发育有关。在COH周期,颗粒细胞AQP9 mRNA表达水平与甾体激素的分泌无相关性。

Expression of aquaporin 9 in granulosa cells of patients with polycystic ovary syndrome in IVF-cycles

Objective:To investigate aquaporin 9(AQP9) mRNA and protein expression in antrum follicle and luteinizing granulosa cells of polycystic ovarian syndrome(PCOS)ovary,and its relation to follicular fluid steroids hormone levels during IVF cycles.Methods: AQP9 mRNA expression on luteinizing granulosa cells in IVF cycles was detected by RT-PCR.AQP9 protein expression in antrum follicles of PCOS ovary and luteinizing granulosa cells was measured by immunhistochemistry.The concentrations of estradiol(E2),progesterone(P) and testerone(T) in follicular fluid were measured by radioimmunoassay(RIA).Results: The expression of AQP9 mRNA in luteinizing granulosa cells during IVF cycles was positive by RT-PCR.No significant differences in AQP9 mRNA levels in granulosa cells between PCOS and control group were found during IVF cycles.The expression level of AQP9 mRNA in large follicles was higher than that in small follicles,but not significantly.The immunoreactivity for AQP9 was localized in membrane and cytoplast of granulosa cells in antrum follicles from PCOS ovary and luteinizing granulosa cells during IVF cycles.Multiple regression analysis showed that AQP9 mRNA levels on granulosa cells were not correlated with E2,P and T levels in follicular fluid during IVF cycles.Conclusion: AQP9 may play an important role in the follicle development and antrum formation through water transport and AQP9 may be involved in the mechanism of follicle development in PCOS.